Effects of Drone Brood Homogenate on Wound Healing: An Experimental Study on Rats.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI:10.1002/vms3.70260

Mehmet Arıcan, Meral Kekecoglu, Yalçın Turhan, Tuğçe Çaprazlı, Mehmet Gamsızkan, Zekeriya Okan Karaduman

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引用次数: 0

Abstract

Background: Wound healing is one of the most interesting topics in orthopaedic surgery, and there are many studies on the factors and mechanisms affecting this process.

Objectives: To evaluated the macroscopic and histopathological results of drone larvae homogenate (DLH) on wound healing in a full-thickness wound model.

Methods: Thirty male wistar rats (6-8 weeks, 250 ± 50 g) were used. A uniform circular full-thickness wound of approximately 18.44 ± 1.45 (control), 19.02 ± 1.24 (silverdin), 19.37 ± 1.28 (DLH) mm² was excised on the back of each rat. They were divided into control (n: 10), silverdin (n: 10) and DLH (n: 10) groups. DLH, collected from the beehive for 3-7 days in late spring and ready for use after homogenization and lyophilization. Two mL of physiological saline, silver sulfadiazine 1% and DLH were applied to the control, silverdin and DLH groups, respectively, and a thin layer that completely covered the wound, and repeated every 2 days for all groups for 14 days. The condition of the lesions was observed every 2 days and the amount of contraction and granulation tissue formed in the lesion was recorded. The lesioned areas was examined histopathologically.

Results: There was no statistically significant difference in lymphocyte, fibroblast, scar thickness, polymorphonuclear leukocyte (PMNL), minivascular density (CD34) and transforming growth factor-β1 (TGF-β1) among the control, silverdin and DLH groups (p = 0.771, 0.434, 0.07, 0.396). The scar density of the DLH group was found to be higher than the control and silverdin groups (p = 0.003). The average wound diameter of the control group (6.87 ± 0.93 mm²) on the 10th day was found to be higher than the silverdin (4.39 ± 1.15 mm²) and DLH groups (4.16 ± 0.55 mm²) (p = 0.0001).

Conclusions: DLH has a positive effect on wound healing, especially by ensuring early wound contraction and wound scar formation.

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来源期刊

Veterinary Medicine and Science Veterinary-General Veterinary

CiteScore

3.00

自引率

0.00%

发文量

296

期刊介绍： Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.