Impact of single nucleotide polymorphism of IL-27P28 rs153109 and IFITM3 rs12252 on susceptibility and severity of COVID-19 in Egyptian patients: a case control study.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-03-08 DOI:10.1186/s12985-025-02668-z

Hanan Hamdy, Reem H Elhamammy, Manal Abdelmageed, Ahmed Wahid

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引用次数: 0

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus disease 2019 (COVID-19), which is a huge global health threat. Interleukin27 (IL-27) gene is a cytokine that produces antiviral proteins in an IFN-independent manner and stimulates both pro- and anti-inflammatory responses. Interferon induced transmembrane protein 3 (IFITM3) inhibits SARS-CoV2 infection by blocking SARSCoV-2 spike proteins which facilitate viral entrance and cell-to-cell fusion. The association between genetic variants and COVID-19 in Egyptians is still unclear. Hence, we sought to investigate the impact of the single nucleotide polymorphism of IL-27P28 rs153109 and IFITM3 rs12252 on the susceptibility and severity of SARS-CoV-2 in Egyptian patients.

Methods: Our study included 242 SARS-CoV-2 patients were recruited from Main University Hospital, Alexandria University, Egypt, and 187 healthy controls. We subdivided the patient group into two subgroups: group A comprised mild/moderate cases (N = 42) (17.4%), and group B included severe/critical cases (N = 200) (82.6%). Genomic DNA was extracted from blood samples using the QIAamp DNA Blood Mini kit, then the PCR products of IL27 and IFITM3 were cut by FastDigest XhoI and MScI, respectively, for detection of SNPs of IL-27P28 rs153109 (-964A/G) and IFITM3 rs12252 (T>C).

Results: The present study found a significant association between IL27 rs153109 (-964A/G) and SARS-CoV-2 infection susceptibility after adjusting for the risk factor (advanced age), IL27 rs153109 (-964A/G) AG genotype (OR = 2.791, 95% CI: 1.237-6.295, P = 0.013), AA genotype (OR = 2.385, 95% CI: 1.075-5.291, P = 0.033), and (AG+AA vs. GG) genotypes (OR = 2.558, 95% CI: 1.186-5.517, P = 0.017). On the other hand, the IFITM3 rs12252(T>C) CT genotype (OR = 1.419, 95% CI: 0.843-2.391, P = 0.188), CC genotype (OR = 2.132, 95% CI: 0.436-10.415, P = 0.350), and (C/T+C/C vs. TT) genotypes (OR = 1.466, 95% CI: 0.884-2.432, P = 0.138) did not show a statistically significant association with either susceptibility or the severity of SARS-CoV-2.

Conclusion: IL27P28 rs153109 AG and AA genotypes of IL27 may be associated with the susceptibility of SARS-CoV-2 infection but not the severity. Concerning the IFITM3 rs12252 SNP, we could not confirm its influence on either susceptibility or the severity of SARS-CoV-2 in this Egyptian population.

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IL-27P28 rs153109和IFITM3 rs12252单核苷酸多态性对埃及患者COVID-19易感性和严重程度的影响：一项病例对照研究

背景：严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）引起的冠状病毒病2019 （COVID-19）是一个巨大的全球健康威胁。白细胞介素27 （IL-27）基因是一种细胞因子，以ifn独立的方式产生抗病毒蛋白，并刺激促炎和抗炎反应。干扰素诱导的跨膜蛋白3 （IFITM3）通过阻断SARSCoV-2刺突蛋白抑制SARS-CoV2感染，SARSCoV-2刺突蛋白促进病毒进入和细胞间融合。遗传变异与埃及人的COVID-19之间的关系尚不清楚。因此，我们试图研究IL-27P28 rs153109和IFITM3 rs12252单核苷酸多态性对埃及患者SARS-CoV-2易感性和严重程度的影响。方法：从埃及亚历山大大学梅因大学医院招募SARS-CoV-2患者242例，健康对照187例。我们将患者组细分为两个亚组：A组包括轻/中度病例（N = 42）（17.4%）， B组包括重/危重病例（N = 200）（82.6%）。使用QIAamp DNA blood Mini试剂盒提取血样中的基因组DNA，然后分别用FastDigest XhoI和MScI切割il -27和IFITM3的PCR产物，检测IL-27P28 rs153109 （-964A/G）和IFITM3 rs12252 （T>C）的snp。结果：本研究发现，在调整危险因素（高龄）、IL27 rs153109 （-964A/G）基因型（OR = 2.791, 95% CI: 1.237 ~ 6.295, P = 0.013）、AA基因型（OR = 2.385, 95% CI: 1.075 ~ 5.291, P = 0.033）和（AG+AA vs. GG）基因型（OR = 2.558, 95% CI: 1.186 ~ 5.517, P = 0.017）后，IL27 rs153109 （-964A/G）与SARS-CoV-2感染易感性存在显著相关性。另一方面，IFITM3 rs12252(T>C) CT基因型（OR = 1.419, 95% CI: 0.843 ~ 2.391, P = 0.188）、CC基因型（OR = 2.132, 95% CI: 0.436 ~ 10.415, P = 0.350）和（C/T+C/C vs. TT）基因型（OR = 1.466, 95% CI: 0.884 ~ 2.432, P = 0.138）与SARS-CoV-2的易感性和严重程度均无统计学意义。结论：IL27P28 rs153109 AG和AA基因型可能与SARS-CoV-2感染的易感性相关，但与严重程度无关。关于IFITM3 rs12252 SNP，我们无法确认其对该埃及人群中SARS-CoV-2易感性或严重程度的影响。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.