Maize SERRATE 1B positively regulates seed germinability under low-temperature

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-03-06 DOI:10.1016/j.plantsci.2025.112458

Qinghui Han , Zan Ren , Qingxiang Zhu , Yang Zhou , Minyi Zhu , Junguang He , Xiaomin Wang , Guangwu Zhao

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引用次数: 0

Abstract

Low temperature poses a significant threat to seedling emergence after maize sowing. While the impact of SERRATE (SE) on plant development via RNA processing has been extensively reported, its involvement in transcriptional regulation or the formation of low-temperature germination ability remains unclear. Our previous research revealed that ZmSE1B is located at the overlapping region of qLTGR4-1 or qLTPRL4-1, which has been associated with low-temperature germination by QTL analysis using IBM Syn4 RIL population. In the present study, we observed that maize seeds overexpressing ZmSE1B exhibited enhanced germination percentages, longer roots, and longer shoots when subjected to low-temperature conditions compared to the wildtype. Through an integrated analysis of RNA-Seq and CUT&Tag, we speculated that ZmGRXCC17, which encodes a GLUTAREDOXIN, may be upregulated by ZmSE1B in maize germinated seeds at low-temperature. Further, the regulation of ZmSE1B on transcription of ZmGRXCC17 was validated using dual-luciferase reporter system and CUT&Tag-qPCR. Finally, the positive effect of ZmGRXCC17 on low-temperature tolerance during seed germination was elucidated through its heterologous expression in rice. The results indicate that ZmSE1B enhances the seed germination ability under low temperature by regulating the transcription of ZmGRXCC17.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.