Allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-03-06 DOI:10.1016/j.plantsci.2025.112453

Liai Ma, Kai Yin, Wenhui Zhu, Yuanbo Wang, Lina Zhang, Ning Yang

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引用次数: 0

Abstract

Abscisic acid (ABA) is a sesquiterpenoid phytohormone involved in controlling plant root growth and development. Thymol, a monoterpene allelochemical, showed a potent phytotoxic effect in plants. It can rapidly inhibit seed germination and seedling growth. In this study, we employed a combination of transcriptome sequencing and validation methods from plant genetics and physiology to investigate the allelopathic inhibitory effects of thymol on the primary roots of Arabidopsis. We found that thymol affected the growth of Arabidopsis thaliana primary root in a dose-dependent manner, low concentration (10 μM) generally enhances, and high concentration (150 μM) inhibits. RNA sequencing analysis showed that a high concentration of thymol affected a series of biological processes and signaling transduction, including ABA biosynthesis, auxin polar transport, oxidative stress, root growth, and development. Exogenous ABA (10 μM) enhanced the inhibitory effect of thymol on the primary root and the application of the ABA biosynthesis inhibitor Na₂WO₄ rescued this inhibitory effect. During this process, the content and distribution of auxin in the roots were significantly altered. The lengths of primary root and meristem of mutant abi1, abi2, and abi1 abi2, showed that ABI1 and ABI2 positively regulate the process of thymol inhibition of root growth. In summary, the allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.