Optimization and comparative study of Bacillus consortia for cellulolytic potential and cellulase enzyme activity.

IF 1.7 4区 生物学 Q3 BIOLOGY
Open Life Sciences Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI:10.1515/biol-2025-1066
Ogechukwu Bose Chukwuma, Mohd Rafatullah, Riti Thapar Kapoor, Husnul Azan Tajarudin, Norli Ismail, Mahboob Alam, Masoom Raza Siddiqui
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引用次数: 0

Abstract

Lignocellulosic biomass, owing to its recalcitrant nature, requires a consortium of enzymes for its breakdown. The present study deals with the isolation of cellulolytic bacterial strains from landfill leachate collected from the Pulau Burung landfill site of Penang, Malaysia, and consortia were constructed to test their cellulolytic efficiency. The dinitro salicylate method was used for the estimation of enzyme activity, and consortia were compared with promising bacterial strains. The combined potential of promising bacterial strains was optimized at varying experimental conditions to detect their maximum cellulolytic activity. The results showed that eight bacterial strains reflected hydrolytic activities, and these were identified by 16S rDNA sequence as Bacillus subtilis, Bacillus pumilus, Bacillus proteolyticus, Bacillus paramycoides, Bacillus cereus, Bacillus altitudinis, Bacillus niacin, and Bacillus thuringiensis. Consortia A included Bacillus proteolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus paramycoides and reflected high thermophilic inclination as the optimal temperature was 45°C at pH 6 with the highest cellulase activity of 0.90 U/ml. Consortia B included Bacillus cereus, Bacillus altitudinis, Bacillus niacin, and Bacillus thuringiensis and showed a cellulase activity of 0.78 U/ml at 38°C and pH 6. The results reflected the significant potential of these Bacillus strains and consortia in the breakdown of cellulose into useful end products. The consortia further proved that a synergistic relationship was more favourable for bioconversion processes.

联合芽孢杆菌纤维素水解潜能和纤维素酶活性的优化与比较研究。
木质纤维素生物质,由于其顽固性,需要一个酶的联盟来分解。本研究从马来西亚槟城布隆岛垃圾填埋场收集的垃圾渗滤液中分离出纤维素降解细菌菌株,并建立菌群以测试其纤维素降解效率。采用二硝基水杨酸法测定酶活性,并与有希望的菌株进行比较。在不同的实验条件下,优化了有希望的菌株的联合电位,以检测它们的最大纤维素水解活性。结果表明,8株菌株具有水解活性,经16S rDNA序列鉴定为枯草芽孢杆菌、矮芽孢杆菌、水解蛋白芽孢杆菌、副芽孢杆菌、蜡样芽孢杆菌、高原芽孢杆菌、烟酸芽孢杆菌和苏云金芽孢杆菌。菌株A包括溶蛋白芽孢杆菌、枯草芽孢杆菌、细芽孢杆菌和副芽孢杆菌,菌株A的最适温度为45℃,pH值为6,纤维素酶活性最高,为0.90 U/ml。菌株B包括蜡样芽孢杆菌、高原芽孢杆菌、烟酸芽孢杆菌和苏云金芽孢杆菌,在38℃、pH 6条件下纤维素酶活性为0.78 U/ml。结果反映了这些芽孢杆菌菌株和联合体在纤维素分解成有用的最终产品方面的巨大潜力。该联合体进一步证明了协同关系更有利于生物转化过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.50
自引率
4.50%
发文量
131
审稿时长
43 weeks
期刊介绍: Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
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