Irisin decreases follicle development in cattle and inhibits theca cell steroidogenesis through focal adhesion kinase signaling.

IF 3.7 3区 生物学 Q1 DEVELOPMENTAL BIOLOGY
Reproduction Pub Date : 2025-03-21 Print Date: 2025-04-01 DOI:10.1530/REP-24-0352
Mathilde Daudon, Christelle Ramé, Marcos H Barreta, Alfredo Q Antoniazzi, Valério M Portela, Europa Meza-Serrano, Joëlle Dupont, Christopher A Price
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Abstract

In brief: Irisin is a muscle and adipose-derived hormone that is secreted in response to negative energy balance in cattle. We show here that irisin reduced follicle growth and theca cell function.

Abstract: At the onset of lactation, dairy cattle are anestrous owing mainly to a state of negative energy balance. Adipose tissue is mobilized to meet the energy demands of milk production, and this alters the secretion of adipose-derived hormones, called adipokines. Irisin is a myokine/adipokine that may play a role in fertility; plasma concentrations increase in cattle postpartum, and irisin decreased progesterone and estradiol secretion from bovine granulosa cells in vitro. To our knowledge, the effects of irisin on bovine theca cell function in vitro and on follicle growth in vivo have not been reported. We hypothesized that irisin negatively affects theca cell function in vitro and causes follicle regression in vivo using well-established bovine models. Under physiological concentrations of insulin (0.2 ng/mL), irisin did not affect glucose uptake, but decreased testosterone secretion and stimulated PTK2 and MTOR phosphorylation. Inhibiting PTK2 activity abolished the ability of irisin to decrease testosterone secretion. Injection of irisin directly into a growing follicle in vivo caused follicle regression. We conclude that irisin decreases bovine theca cell steroidogenesis through PTK2 signaling, and combined effects on theca and granulosa cells cause follicle regression.

鸢尾素通过局灶黏附激酶信号传导降低牛卵泡发育并抑制卵泡细胞甾体生成。
泌乳期初期,奶牛处于负能量平衡状态,处于发情状态。脂肪组织被动员起来满足产奶的能量需求,这改变了脂肪衍生激素的分泌,称为脂肪因子。鸢尾素是一种可能在生育中发挥作用的肌肉因子/脂肪因子;产后牛血浆浓度升高,鸢尾素降低体外牛颗粒细胞的孕酮和雌二醇分泌。据我们所知,鸢尾素对体外牛卵泡细胞功能和体内卵泡生长的影响尚未见报道。我们假设鸢尾素在体外对卵泡细胞功能产生负面影响,并在体内引起卵泡退化,使用了成熟的牛模型。在胰岛素生理浓度(0.2 ng/mL)下,鸢尾素不影响葡萄糖摄取,但降低睾酮分泌,刺激PTK2和mTOR磷酸化。抑制PTK2活性可消除鸢尾素降低睾酮分泌的能力。在体内将鸢尾素直接注射到生长中的卵泡中,可引起卵泡退化。我们得出结论,鸢尾素通过PTK2信号传导降低了牛乳膜细胞的类固醇生成,并且对乳膜细胞和颗粒细胞的联合作用导致了卵泡退化。
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来源期刊
Reproduction
Reproduction 生物-发育生物学
CiteScore
7.40
自引率
2.60%
发文量
199
审稿时长
4-8 weeks
期刊介绍: Reproduction is the official journal of the Society of Reproduction and Fertility (SRF). It was formed in 2001 when the Society merged its two journals, the Journal of Reproduction and Fertility and Reviews of Reproduction. Reproduction publishes original research articles and topical reviews on the subject of reproductive and developmental biology, and reproductive medicine. The journal will consider publication of high-quality meta-analyses; these should be submitted to the research papers category. The journal considers studies in humans and all animal species, and will publish clinical studies if they advance our understanding of the underlying causes and/or mechanisms of disease. Scientific excellence and broad interest to our readership are the most important criteria during the peer review process. The journal publishes articles that make a clear advance in the field, whether of mechanistic, descriptive or technical focus. Articles that substantiate new or controversial reports are welcomed if they are noteworthy and advance the field. Topics include, but are not limited to, reproductive immunology, reproductive toxicology, stem cells, environmental effects on reproductive potential and health (eg obesity), extracellular vesicles, fertility preservation and epigenetic effects on reproductive and developmental processes.
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