Multi-stimuli-responsive pectin-coated dendritic mesoporous silica nanoparticles with Eugenol as a sustained release nanocarrier for the control of tomato bacterial wilt.

IF 10.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xueping Guo, Huiyan Li, Zhihao Li, Ziqi Cui, Guangming Ma, Aisha Khalfan Nassor, Yi Guan, Xiaohong Pan
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引用次数: 0

Abstract

Background: Environmentally responsive nanoscale biocide delivery system enhances smart, regulated, and synergistic biocide application with precise biocide release. In this study, pectin-modified dendritic mesoporous silica nanoparticles (DMSNs) was used as a carrier to successfully construct a microenvironment-responsive (pH, temperature and enzyme) eugenol nano-biocide delivery system for the control of Ralstonia solanacearum infection.

Results: The results showed that the specific surface area, pore size and surface activity of DMSNs significantly influence the biocide loading of eugenol, and the biocide loading capability was up to 72.50%. Eu@DMSNs/Pec had significant pH and pectinase stimulating effects, with varying release amounts under different temperature conditions. Compared with eugenol alone, Eu@DMSNs/Pec significantly enhanced the efficacy of eugenol. DMSNs assisted eugenol to induce peroxidation damage, produce ROS (•O2-, •OH and 1O2), achieve synergistic antibacterial effects, and had better rain erosion resistance and foliar retention rate based on pectin wettability and adhesion. Eu@DMSNs/Pec-FITC showed demonstrated efficient transport characteristics in tomato roots, stems and leaves, which enhanced the control effect on tomato bacterial wilt. In addition, Eu@DMSNs/Pec exert minimal influence on tomato seed germination and root growth, and have low toxicity to non-target organisms such as earthworms. Therefore, Eu@DMSNs/Pec environment-responsive nano-controlled release nanocarrier can effectively achieve accurate biocide release and reduce biocide dosage.

Conclusion: This work not only provides a pectin-modified DMSNs-based eugenol nanoscale biocide delivery system in response to specific environmental conditions of R. solanacearum infection but also elucidates the eugenol biocide loading, selective release ability and antibacterial mechanism of the system.

以丁香酚为缓释载体的果胶包覆枝状介孔二氧化硅纳米颗粒防治番茄青枯病的研究。
背景:环境敏感的纳米级杀菌剂输送系统通过精确的杀菌剂释放,增强了杀菌剂的智能、调节和协同应用。本研究以果胶修饰的树突状介孔二氧化硅纳米颗粒(DMSNs)为载体,成功构建了一种微环境响应(pH、温度和酶)的丁香酚纳米杀菌剂递送系统,用于控制茄枯菌的感染。结果:dmsn的比表面积、孔径和表面活性对丁香酚的杀菌剂负载有显著影响,杀菌剂负载率可达72.50%。Eu@DMSNs/Pec具有显著的pH和刺激果胶酶的作用,不同温度条件下的释放量不同。与单独使用丁香酚相比,Eu@DMSNs/Pec显著增强了丁香酚的功效。DMSNs辅助丁香酚诱导过氧化损伤,产生活性氧(•O2-、•OH和1O2),达到协同抗菌作用,并具有较好的抗雨蚀性和基于果胶润湿性和附着力的叶面保有率。Eu@DMSNs/Pec-FITC在番茄根、茎、叶中表现出高效的转运特性,增强了对番茄青枯病的防治效果。此外,Eu@DMSNs/Pec对番茄种子萌发和根系生长的影响很小,对蚯蚓等非目标生物的毒性较低。因此,Eu@DMSNs/Pec环境响应型纳米缓释纳米载体可以有效实现杀菌剂的精准释放,减少杀菌剂用量。结论:本研究不仅提供了一种基于果胶修饰dmsns的丁香酚纳米级杀菌剂递送体系,以应对茄青霉感染的特定环境条件,而且阐明了该体系的丁香酚杀菌剂负载、选择性释放能力和抗菌机制。
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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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