Effect of betaine on growth performance, methionine metabolism, and methyl transfer in broilers aged 1 to 21 days and fed a low-methionine diet.

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Poultry Science Pub Date : 2025-03-07 eCollection Date: 2025-01-01 DOI:10.2141/jpsa.2025010
Heng Wang, Lin Liu, Xi He, Guozhi Bian
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引用次数: 0

Abstract

Betaine has been proposed as a low-cost source of methyl groups in poultry feed, replacing methionine and choline. The present study aimed to investigate the effect of betaine on growth performance, methionine metabolism, and methyl transfer in broilers aged 1 to 21 days fed a low-methionine diet. A total of 960 one-day-old male broilers were randomly divided into four groups: positive control (0.62% methionine in the diet), negative control (0.37% methionine in the diet), and two treatment groups (0.37% methionine in the diet plus either 1500 or 3000 mg betaine/kg diet). Chicks fed the 1500 mg betaine/kg diet had the highest feed-to-gain ratio (P < 0.05), but no significant difference in final body weight, average daily gain, average daily feed intake, or mortality. Serum S-adenosyl-L-methionine and total homocysteine were higher at 1500 mg betaine/kg diet; whereas serum S-adenosylhomocysteine exhibited the opposite trend. Except for DNA methyltransferase 1, key enzymes and metabolites involved in the hepatic single-carbon pathway showed the highest levels at 1500 mg betaine/kg diet and declined thereafter. Furthermore, betaine promoted dose-dependent mRNA and protein expression of enzymes involved in the hepatic single-carbon metabolic cycle and methyl transferase pathways in chicks fed methionine-deficient diets. In conclusion, while the addition of betaine did not significantly improve the growth performance of chicks aged 1-21 days, inclusion of 1500 mg betaine/kg diet effectively stabilized methionine metabolism and methyl transfer in methionine-deficient diets.

甜菜碱对低蛋氨酸饲粮1 ~ 21日龄肉仔鸡生长性能、蛋氨酸代谢和甲基转移的影响
甜菜碱已被提出作为家禽饲料中甲基的低成本来源,以取代蛋氨酸和胆碱。本试验旨在研究甜菜碱对1 ~ 21日龄低蛋氨酸饲粮肉鸡生长性能、蛋氨酸代谢和甲基转移的影响。选取960只1日龄雄性肉仔鸡,随机分为4组:阳性对照组(饲粮中蛋氨酸含量为0.62%)、阴性对照组(饲粮中蛋氨酸含量为0.37%)和2个处理组(饲粮中蛋氨酸含量为0.37% +甜菜碱1500或3000 mg /kg)。1500 mg甜菜碱/kg饲粮的料重比最高(P < 0.05),但对末重、平均日增重、平均日采食量和死亡率无显著影响。1500 mg甜菜碱/kg饲粮时血清s -腺苷- l-蛋氨酸和总同型半胱氨酸较高;血清s -腺苷型同型半胱氨酸呈相反趋势。除DNA甲基转移酶1外,肝脏单碳途径相关的关键酶和代谢产物在1500 mg甜菜碱/kg饲粮时水平最高,此后呈下降趋势。此外,甜菜碱促进了蛋氨酸缺乏雏鸡肝脏单碳代谢循环和甲基转移酶途径相关酶的mRNA和蛋白表达。由此可见,添加甜菜碱对1 ~ 21日龄雏鸡生长性能没有显著改善,但在蛋氨酸缺乏饲粮中添加1500 mg甜菜碱可有效稳定蛋氨酸代谢和甲基转移。
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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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