Evaluation of SIRT1 Protein Levels and SIRT1/rs7895833 Distributions in Turkish Patients With Obstructive Sleep Apnea.

IF 2.6 4区 医学 Q2 MEDICAL LABORATORY TECHNOLOGY
Metehan Yaman, Merve Nur Yıldız, Fatih Atilla Bağcı, Ceren Gümüş, Ahmet Ursavaş, Mehmet Karadağ, Dilek Pirim
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引用次数: 0

Abstract

Objective: Obstructive Sleep Apnea (OSA) is a common heterogeneous sleep disorder that significantly impacts the sleep quality of individuals and leads to severe complications. Patients with OSA often experience disrupted circadian rhythm, hyperactive hypoxia response, and endothelial dysfunction, yet the underlying molecular mechanism remains poorly known. Recent research suggests promising evidence of the potential role of SIRT1 in the etiology of OSA, warranting further investigation.

Methods: We investigated the associations of the SIRT1 promoter variant (rs7895833A > G) with OSA severity in 199 individuals who underwent an overnight polysomnography at the sleep clinic.

Results: The minor allele frequency was observed as 0.309 in males (n = 149) and 0.310 in females (n = 50). No significant associations were observed between genotypes and apnea-hypopnea index (AHI) in the entire sample. However, we observed a significant association (p = 0.034) between the rs7895833-G and the severity of OSA in females stratified by AHI. Additionally, we found statistically significant inverse correlations between age and SIRT1 protein levels in the total sample (p = 0.013) and the male group (p = 0.018), suggesting a potential age-related expression of SIRT1. Our analysis also confirmed the published literature, showing correlations between the AHI and clinical parameters such as age, BMI, Epworth sleepiness scale, and neck circumference.

Conclusions: Overall, SIRT1 may indirectly affect OSA pathogenesis, which might be influenced by gender. Further detailed analysis involving large population-based biobanks, especially focusing on gender-based differences, will improve our understanding of the role and potential of SIRT1 in OSA management.

评估土耳其阻塞性睡眠呼吸暂停患者的 SIRT1 蛋白水平和 SIRT1/rs7895833 分布。
目的:阻塞性睡眠呼吸暂停(OSA)是一种常见的异质性睡眠障碍,严重影响个人的睡眠质量,并导致严重的并发症。OSA 患者通常会出现昼夜节律紊乱、低氧反应亢进和内皮功能障碍,但其潜在的分子机制仍鲜为人知。最近的研究表明,SIRT1 在 OSA 的病因学中具有潜在作用,值得进一步研究:方法:我们调查了在睡眠诊所接受过夜间多导睡眠图检查的 199 人中 SIRT1 启动子变异(rs7895833A > G)与 OSA 严重程度的关系:男性(149 人)和女性(50 人)的小等位基因频率分别为 0.309 和 0.310。在所有样本中,未观察到基因型与呼吸暂停-低通气指数(AHI)之间有明显关联。但是,我们观察到,在按 AHI 分层的女性中,rs7895833-G 与 OSA 的严重程度有明显关联(p = 0.034)。此外,我们还发现在全部样本(p = 0.013)和男性组(p = 0.018)中,年龄与 SIRT1 蛋白水平之间存在统计学意义上的显著反相关性,这表明 SIRT1 的表达可能与年龄有关。我们的分析还证实了已发表的文献,显示 AHI 与年龄、体重指数、埃普沃斯嗜睡量表和颈围等临床参数之间存在相关性:总体而言,SIRT1 可能会间接影响 OSA 的发病机制,而这可能会受到性别的影响。通过对大型人群生物库进行进一步的详细分析,尤其是关注性别差异,将有助于我们更好地了解 SIRT1 在 OSA 治疗中的作用和潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Clinical Laboratory Analysis
Journal of Clinical Laboratory Analysis 医学-医学实验技术
CiteScore
5.60
自引率
7.40%
发文量
584
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.
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