Evaluation of immunological effects of two DNA vaccines against Nocardia seriolae in hybrid snakehead.

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-03-07 DOI:10.1016/j.fsi.2025.110233

Yiming Wen, Guoquan Chen, Yuhao Li, Deyu Ning, Wanna Sirimanapong, Liqun Xia

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Abstract

Although DNA vaccines hold significant potential, their practical application in aquaculture remains limited. In both mammals and teleost fish, B cells, which recognize antigens and produce antibodies, play an important role in immunity. In this study, B-cell epitopes capable of inducing fish immunity from IS701 family transposase (IS701) and molybdopterin-dependent oxidoreductase (Mol) proteins of Nocardia seriolae were screened. pcDNA-IS701 and pcDNA-Mol recombinant plasmids were constructed. The results showed that two proteins possessed multiple B-cell epitopes, and both pcDNA-IS701 and pcDNA-Mol induced innate immunity and specific antibody responses, along with increased mRNA expression levels of genes encoding humoral (MHCIIα and CD4) and cell-mediated (MHCIα and CD8α) immunity. In addition, both pcDNA-IS701 and pcDNA-Mol strengthened the protection against N. seriolae infection, with immune protection rates of 45.06% for IS701 and 61.04% for Mol, respectively. In conclusion, pcDNA-IS701 and pcDNA-Mol are candidate DNA vaccines for nocardiosis in fish.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.