Rutin resists Aspergillus fumigatus keratitis by activating Nrf2/HO-1 pathway, inhibiting Dectin-1/p-Syk pathway and affecting fungal structures

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-03-05 DOI:10.1016/j.exer.2025.110323

Yuqi Li , Xue Tian , Lina Zhang, Jing Lin, Qian Wang, Lingwen Gu, Hong Li, Bing Yu, Ziyi Wang, Menghui Chi, Guiqiu Zhao, Cui Li

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引用次数: 0

Abstract

Fungal keratitis (FK) is a severe vision-threatening eye disease. The fungal invasiveness and excessive inflammatory response contribute to corneal tissue damage. Rutin (RT) possesses anti-inflammatory, antimicrobial, antioxidant, and improved wound-healing characteristics. This study aimed to evaluate antifungal, anti-inflammatory, and therapeutic effects of RT in FK. The results showed that RT exerted antifungal effects by inhibiting fungal growth, altering hyphal morphology, destroying biofilm, and disrupting fungal cellular structures. RT exhibited anti-inflammatory benefits by suppressing the Dectin-1/p-Syk pathway, activating the Nrf2/HO-1 pathway, and decreasing the expression of inflammatory factors in vivo and in vitro. RT demonstrated therapeutic effects by reducing clinical scores, fungal load, macrophage recruitment, and neutrophil activity. In conclusion, RT exhibited anti-inflammatory, antifungal, and therapeutic effects in Aspergillus fumigatus keratitis, and has the potential to become a novel therapeutic strategy for FK.

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芦丁通过激活Nrf2/HO-1通路、抑制Dectin-1/p-Syk通路、影响真菌结构等途径抵抗烟曲霉角膜炎。

真菌性角膜炎（FK）是一种严重威胁视力的眼病。真菌的侵袭性和过度的炎症反应导致角膜组织损伤。芦丁（RT）具有抗炎、抗菌、抗氧化和改善伤口愈合的特性。本研究旨在评价RT对FK的抗真菌、抗炎和治疗作用。结果表明，RT通过抑制真菌生长、改变菌丝形态、破坏生物膜和破坏真菌细胞结构发挥抑菌作用。RT通过抑制Dectin-1/p-Syk通路，激活Nrf2/HO-1通路，降低体内外炎症因子的表达，显示出抗炎作用。RT通过降低临床评分、真菌负荷、巨噬细胞募集和中性粒细胞活性显示出治疗效果。综上所述，RT在烟曲霉角膜炎中具有抗炎、抗真菌和治疗作用，有可能成为一种新的治疗FK的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.