A modified formulation of Zoono GermFree24 antiseptic liquid has enhanced efficacy against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biofouling Pub Date : 2025-03-01 Epub Date: 2025-03-09 DOI:10.1080/08927014.2025.2467078
Samuel J M Hale, Christian A Lux, Raymond Kim, Kristi Biswas, Brett Wagner Mackenzie, Richard G Douglas
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引用次数: 0

Abstract

Zoono GermFree24 is a quaternary ammonium compound-based hand sanitiser. Its efficacy against planktonic bacteria is well established, but efficacy against biofilms has not been tested. We investigated the antibiofilm efficacy of Zoono GermFree24 hand sanitiser and a modified formulation against biofilms of Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 grown in vitro using the Centers for Disease Control (CDC) biofilm reactor and 96-pin lids. Biofilms were immersed in Zoono GermFree24 or Zoono B22-1402A (modified formulation) for 5 min, 1 h, 6 h or overnight (22 ± 2 h). The antiseptic was neutralised and the bacteria remaining after treatment were cultured and quantified. Zoono GermFree24 and Zoono B22-1402A caused time-dependent reductions in viable biofilms of both species with both methods of culture and testing, with more rapid biofilm eradication observed for Zoono B22-1402A. Biofilms grown on 96-pin lids were more quickly eradicated than those grown in the CDC biofilm reactor.

改良配方的Zoono gerfree24抗菌液对金黄色葡萄球菌和铜绿假单胞菌的生物膜具有增强的功效。
Zoono gerfree24是一种季铵化合物基洗手液。它对浮游细菌的功效已得到证实,但对生物膜的功效尚未经过测试。采用美国疾病控制与预防中心(CDC)生物膜反应器和96针盖,研究了Zoono gerfree24洗手液及其改良配方对体外培养的金黄色葡萄球菌ATCC 6538和铜绿假单胞菌ATCC 27853生物膜的抗菌效果。将生物膜浸泡在Zoono GermFree24或Zoono B22-1402A(改良配方)中5分钟、1小时、6小时或过夜(22±2小时)。对灭菌剂进行中和,并对处理后残存的细菌进行培养和定量。Zoono gerfree24和Zoono B22-1402A在培养和测试两种方法下均引起两种物种活膜的时间依赖减少,其中Zoono B22-1402A的生物膜清除速度更快。在96针盖上生长的生物膜比在CDC生物膜反应器中生长的生物膜根除得更快。
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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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