Pelvic vasectomy and its protective effects on rat testis function.

IF 2.4 3区 医学 Q2 ANDROLOGY
Heng Yang, Yujun Chen, Xiaofeng Cheng, Jingxin Wu, Ruohui Huang, Biao Qian, Gongxian Wang
{"title":"Pelvic vasectomy and its protective effects on rat testis function.","authors":"Heng Yang, Yujun Chen, Xiaofeng Cheng, Jingxin Wu, Ruohui Huang, Biao Qian, Gongxian Wang","doi":"10.1186/s12610-025-00255-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vasectomy is a commonly used male contraceptive method, but the choice of surgical technique can influence long-term reproductive health outcomes. Previous studies suggest that different vasectomy techniques may lead to varying degrees of tissue damage, oxidative stress, and endocrine dysfunction. However, there is limited research on how these techniques affect overall reproductive system function. Therefore, this study aims to evaluate and compare the effects of two vasectomy techniques on reproductive system parameters in rats.</p><p><strong>Methods: </strong>Twenty-four specific pathogen-free male Sprague-Dawley rats weighing 250-300 g were randomly divided into four groups: sham operation group, negative control group, traditional vasectomy group, and modified vasectomy group, with six rats in each group. Each group underwent specific vasectomy procedures, followed by a three-month recovery period. Experimental methods included hematoxylin and eosin staining, immunohistochemistry in the epididymis of rats, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling for apoptosis detection, enzyme-linked immunosorbent assay for measuring serum hormone and oxidative stress markers, as well as tests for sexual behavior and anxiety-like behavior.</p><p><strong>Results: </strong>The modified vasectomy group exhibited improved epididymis morphology compared to the traditional vasectomy group. Immunohistochemistry demonstrated reduced levels of apoptosis in the modified vasectomy group, which was further corroborated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining, indicating lower cell death. Hormone analysis revealed stable levels in the modified vasectomy group, and oxidative stress markers indicated reduced stress responses. Behavioral test assessing sexual activity and anxiety level was consistent with these findings.</p><p><strong>Conclusion: </strong>Modified vasectomy techniques provide superior protection of reproductive system functionality in rats compared to traditional methods. These techniques reduce tissue damage, cell apoptosis, and oxidative stress while maintaining endocrine function, offering promising implications for clinical applications.</p>","PeriodicalId":8730,"journal":{"name":"Basic and Clinical Andrology","volume":"35 1","pages":"9"},"PeriodicalIF":2.4000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889833/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and Clinical Andrology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12610-025-00255-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANDROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Vasectomy is a commonly used male contraceptive method, but the choice of surgical technique can influence long-term reproductive health outcomes. Previous studies suggest that different vasectomy techniques may lead to varying degrees of tissue damage, oxidative stress, and endocrine dysfunction. However, there is limited research on how these techniques affect overall reproductive system function. Therefore, this study aims to evaluate and compare the effects of two vasectomy techniques on reproductive system parameters in rats.

Methods: Twenty-four specific pathogen-free male Sprague-Dawley rats weighing 250-300 g were randomly divided into four groups: sham operation group, negative control group, traditional vasectomy group, and modified vasectomy group, with six rats in each group. Each group underwent specific vasectomy procedures, followed by a three-month recovery period. Experimental methods included hematoxylin and eosin staining, immunohistochemistry in the epididymis of rats, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling for apoptosis detection, enzyme-linked immunosorbent assay for measuring serum hormone and oxidative stress markers, as well as tests for sexual behavior and anxiety-like behavior.

Results: The modified vasectomy group exhibited improved epididymis morphology compared to the traditional vasectomy group. Immunohistochemistry demonstrated reduced levels of apoptosis in the modified vasectomy group, which was further corroborated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining, indicating lower cell death. Hormone analysis revealed stable levels in the modified vasectomy group, and oxidative stress markers indicated reduced stress responses. Behavioral test assessing sexual activity and anxiety level was consistent with these findings.

Conclusion: Modified vasectomy techniques provide superior protection of reproductive system functionality in rats compared to traditional methods. These techniques reduce tissue damage, cell apoptosis, and oxidative stress while maintaining endocrine function, offering promising implications for clinical applications.

盆腔输精管切除术及其对大鼠睾丸功能的保护作用。
背景:输精管切除术是一种常用的男性避孕方法,但手术技术的选择会影响长期的生殖健康结果。以往的研究表明,不同的输精管结扎技术可能导致不同程度的组织损伤、氧化应激和内分泌功能障碍。然而,关于这些技术如何影响生殖系统整体功能的研究有限。因此,本研究旨在评价和比较两种输精管结扎技术对大鼠生殖系统参数的影响。方法:选取24只体重250 ~ 300 g的雄性无特异性病原体Sprague-Dawley大鼠,随机分为假手术组、阴性对照组、传统输精管结扎组和改良输精管结扎组,每组6只。每组都进行了特定的输精管切除术,随后是三个月的恢复期。实验方法包括苏木精和伊红染色、大鼠附睾免疫组化、末端脱氧核苷酸转移酶介导的dUTP镍端标记检测细胞凋亡、酶联免疫吸附法检测血清激素和氧化应激标志物、性行为和焦虑样行为测试。结果:改良输精管结扎术组与传统输精管结扎术组相比,附睾形态有所改善。免疫组织化学显示改良输精管切除术组细胞凋亡水平降低,末端脱氧核苷酸转移酶介导的dUTP镍端标记染色进一步证实了这一点,表明细胞死亡率降低。激素分析显示改良输精管结扎组水平稳定,氧化应激标志物显示应激反应降低。评估性活动和焦虑水平的行为测试与这些发现一致。结论:改良输精管结扎技术对大鼠生殖系统功能的保护作用优于传统方法。这些技术在维持内分泌功能的同时减少了组织损伤、细胞凋亡和氧化应激,具有良好的临床应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Basic and Clinical Andrology
Basic and Clinical Andrology Medicine-Urology
CiteScore
3.50
自引率
0.00%
发文量
21
审稿时长
22 weeks
期刊介绍: Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信