Non-transgenic RNAi strategies for sustainable plant viral disease control: a review.

Abstract

Viruses are a major cause of plant disease with an estimated annual economic impact of over $30 billion. They account for nearly 50% of the pathogens responsible for emerging and re-emerging plant diseases worldwide. To confer resistance against these diseases RNA interference (RNAi) technology can be employed. Designing silencing molecules like dsRNA homologous to the viral genome has been the most common non-transgenic method to induce RNAi mediated resistance in plants. dsRNAs are carefully tailored and produced considering factors such as the type of virus, target genomic region, dsRNA size, and method of application to maximise their efficiency. With the advent of new technologies like nano-platforms, a sustainable carrier can be developed to deliver these molecules, enhancing their stability and its bioavailability. This innovative technology faces regulatory debates globally and lacks legislation for commercialisation. But this is an eco-friendly alternative to conventional pesticides that can revolutionize the future of plant viral disease management, providing a bio-safe and an evergreen solution.