Identification of LIMK1 as a biomarker in clear cell renal cell carcinoma: from data mining to validation.

IF 2.7 3区医学 Q3 ONCOLOGY

Journal of Cancer Research and Clinical Oncology Pub Date : 2025-03-08 DOI:10.1007/s00432-025-06146-5

Yifei Li, Congcong Fan, Feng Jiang, Jingnan Zhang, Yanzhen Li, Yanjie Jiang, Rui Zhang, Zhixian Yu, Siqi Wang

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引用次数: 0

Abstract

Purpose: Clear cell renal cell carcinoma (ccRCC) is one of the most common types of renal cancer. LIM kinase 1 (LIMK1) reportedly plays an important role in tumorigenesis. However, the involvement of LIMK1 in the progression of ccRCC remains ambiguous.

Methods: Based on the TCGA and CPTAC databases, the expression of LIMK1 in ccRCC was evaluated. In the TCGA-ccRCC cohort, the relationships between LIMK1 and immune cell infiltration as well as immune checkpoints were assessed. The high expression of LIMK1 in ccRCC was verified by qRT-PCR in four RCC cell lines. Immunohistochemistry was used to evaluate the expression of LIMK1 in clinical samples. The association between LIMK1 expression and survival prognosis was explored via Kaplan-Meier survival curve in the TCGA-ccRCC and local cohorts. The effects of LIMK1 knockdown on the proliferation, migration, and invasion abilities of RCC cells were evaluated via colony, CCK-8, wound healing, and Transwell assays.

Results: Elevated expression level of LIMK1 was found in the TCGA-ccRCC cohort and was confirmed in RCC cell lines and clinical samples. Up-regulation of LIMK1 was found to be correlated with poor prognosis in TCGA-ccRCC and external cohorts. In addition, high-LIMK1 was associated with clinicopathological stage, immune cell infiltration and immune checkpoint in ccRCC. Importantly, knockdown of LIMK1 diminished the capability of proliferation, migration, and invasion in RCC cells.

Conclusion: LIMK1 may serve as a promising diagnostic and prognostic biomarker of ccRCC.

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LIMK1作为透明细胞肾细胞癌生物标志物的鉴定：从数据挖掘到验证。

目的：透明细胞肾细胞癌（Clear cell renal cell carcinoma, ccRCC）是最常见的肾癌类型之一。据报道，LIM激酶1 （LIMK1）在肿瘤发生中起重要作用。然而，LIMK1参与ccRCC的进展仍然不清楚。方法：基于TCGA和CPTAC数据库，评估LIMK1在ccRCC中的表达。在TCGA-ccRCC队列中，评估了LIMK1与免疫细胞浸润以及免疫检查点之间的关系。在4个RCC细胞系中，qRT-PCR证实了LIMK1在ccRCC中的高表达。免疫组化法检测LIMK1在临床样品中的表达。在TCGA-ccRCC和本地队列中，通过Kaplan-Meier生存曲线探讨LIMK1表达与生存预后的关系。通过集落、CCK-8、伤口愈合和Transwell试验评估LIMK1敲低对RCC细胞增殖、迁移和侵袭能力的影响。结果：LIMK1在TCGA-ccRCC队列中表达水平升高，并在RCC细胞系和临床样本中得到证实。在TCGA-ccRCC和外部队列中发现LIMK1上调与预后不良相关。此外，高limk1与ccRCC的临床病理分期、免疫细胞浸润和免疫检查点相关。重要的是，LIMK1的敲低降低了RCC细胞的增殖、迁移和侵袭能力。结论：LIMK1有望作为ccRCC的诊断和预后生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cancer Research and Clinical Oncology 医学-肿瘤学

CiteScore

4.00

自引率

2.80%

发文量

577

审稿时长

2 months

期刊介绍： The "Journal of Cancer Research and Clinical Oncology" publishes significant and up-to-date articles within the fields of experimental and clinical oncology. The journal, which is chiefly devoted to Original papers, also includes Reviews as well as Editorials and Guest editorials on current, controversial topics. The section Letters to the editors provides a forum for a rapid exchange of comments and information concerning previously published papers and topics of current interest. Meeting reports provide current information on the latest results presented at important congresses. The following fields are covered: carcinogenesis - etiology, mechanisms; molecular biology; recent developments in tumor therapy; general diagnosis; laboratory diagnosis; diagnostic and experimental pathology; oncologic surgery; and epidemiology.