Accumulation of heavy metals in the mycelium of Abortiporus biennis and their effect on oxalate oxidase activity

IF 4.1 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biometals Pub Date : 2025-03-08 DOI:10.1007/s10534-025-00675-9

Marcin Grąz, Anna Jarosz-Wilkołazka, Jolanta Polak, Bożena Pawlikowska-Pawlęga, Justyna Kapral-Piotrowska, Wioleta Ciempiel, Marta Ruminowicz-Stefaniuk

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Abstract

Abortiporus biennis belongs to a basidiomycete fungi with the unique ability to degrade oxalate using enzyme oxalate oxidase. Oxalate oxidase (OXO) from Abortiporus biennis is an intracellular oxalic acid-induced enzyme, which catalyses the degradation of oxalic acid to carbon dioxide and hydrogen peroxide. OXO activity was stimulated by all the heavy metals tested (copper, manganese, lead and cadmium) after addition as water-soluble salts to A. biennis fungal cultures. Manganese ions stimulated OXO activity to the greatest extent among the tested metal ions. In the presence of manganese ions, an elevated concentration of oxalate was also detected in the fungal culture after 7 days of cultivation. The manganese, lead, and cadmium ions were accumulated by the A. biennis mycelium. Among them, lead was accumulated to a great extent. It was observed that the accumulation of lead ions occurred in the vicinity of the A. biennis hyphal cell wall.

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双生Abortiporus biennis菌丝中重金属的积累及其对草酸氧化酶活性的影响。

流产孢子属担子真菌，具有利用草酸氧化酶降解草酸盐的独特能力。草酸氧化酶（OXO）是一种细胞内草酸诱导酶，可催化草酸降解为二氧化碳和过氧化氢。所有重金属（铜、锰、铅和镉）作为水溶性盐添加到双叶曲霉真菌培养物中后，都能刺激OXO活性。锰离子对氧化氧活性的刺激作用最大。在锰离子存在的情况下，真菌培养7天后也检测到草酸盐浓度升高。锰离子、铅离子和镉离子通过双生藤菌丝体积累。其中，铅在很大程度上是积累的。结果表明，铅离子的积累主要发生在双歧杆菌菌丝细胞壁附近。

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来源期刊

Biometals 生物-生化与分子生物学

CiteScore

5.90

自引率

8.60%

发文量

111

审稿时长

3 months

期刊介绍： BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.