Synthesis of trypsin-protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-03-10 DOI:10.1007/s00604-025-07070-8

Suresh Kumar Kailasa, Kartik Pankajbhai Makwana, Madhura Pradeep Deshpande, Yoojin Choi, Ruth Stephanie, Chan Yeong Park, Tae Jung Park

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引用次数: 0

Abstract

Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for molecular species sensing in aqueous media. Here, we introduce trypsin (Try) as a bioligand for the synthesis of cesium lead chloride (CsPbCl₃) PNCs with high water stability. The as-fabricated Try-CsPbCl₃ PNCs show λ_Em/Ex at 433/370 nm with a quantum yield of 17.26%. The fluorescence emission spectral characteristics of Try-CsPbCl₃ PNCs demonstrated that water-stable Try-CsPbCl₃ PNCs acted as a promising fluorescent probe for the detection of hydroxyl radical (^•OH) via turn-off mechanism. The Try-CsPbCl₃ PNCs-based turn-off fluorescence approach displayed good selectivity for hydroxyl radical in water, showing a wider linear range (0.01–5 µM) with a remarkable detection limit of 3.10 nM for hydroxyl radical. The Try-CsPbCl₃ PNCs were demonstrated to be a facile probe for sensing ^•OH in water samples, which signifies that Try-CsPbCl₃ PNCs exhibited broad applications for hydroxyl radical sensing in real samples.

Graphical Abstract

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用于羟基自由基传感的胰蛋白酶保护CsPbCl3荧光纳米晶体的合成

水分散钙钛矿纳米晶体（PNCs）由于其优异的光学性能在离子和分子识别方面具有广阔的应用前景。然而，当卤化铅pnc用作水介质中分子种类传感的探针时，它们存在一些局限性。在这里，我们引入胰蛋白酶（Try）作为合成具有高水稳定性的氯化铯铅（CsPbCl3） pnc的生物配体。制备的Try-CsPbCl3 pnc在433/370 nm处表现出λEm/Ex，量子产率为17.26%。通过对其荧光发射光谱特性的研究，表明水稳定性较好的Try-CsPbCl3 pnc可作为一种通过关闭机制检测羟基自由基（•OH）的荧光探针。基于Try-CsPbCl3 pncs的关闭荧光方法对水中羟基自由基具有良好的选择性，具有较宽的线性范围（0.01 ~ 5µM），对羟基自由基的检测限为3.10 nM。实验证明，Try-CsPbCl3 pnc是一种易于检测水样中•OH的探针，这表明Try-CsPbCl3 pnc在实际样品中具有广泛的羟基自由基检测应用。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.