Characterization of the ribosomal RNA methylase gene erm(A) and its promoter mutation in Campylobacter coli from chicken cecum

IF 5 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

International journal of food microbiology Pub Date : 2025-03-05 DOI:10.1016/j.ijfoodmicro.2025.111138

Ting Liu , Xiao Liu , Xingyang Dai , Jinhu Huang , Runsheng Li , Liping Wang , Xiaoming Wang

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引用次数: 0

Abstract

Macrolide antibiotics are commonly used to treat campylobacteriosis in both clinical settings and animal husbandry. The emergence of macrolide-resistant Campylobacter poses public health risks. In this study, six Campylobacter coli strains carrying erm(A) gene were identified from chicken cecum samples, and the functionality and genetic environment of erm(A) were analyzed. Antimicrobial susceptibility testing was conducted on Campylobacter isolates. WGS was used to analyze the genetic characteristics of erm(A)-positive strains. Cloning and in vitro-induced drug resistance assay were used to investigate the function of erm(A) gene. A total of 42 (20.2 %) C. coli isolates were obtained from 208 samples collected from chicken cecum, among which 6 erm(A)-positive. Induced drug resistance assay revealed a cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increase in erythromycin MIC. WGS analysis showed that the erm(A) gene in C. coli shared high nucleotide sequence identity with that in Enterococcus faecalis and co-located with optrA between IS1216. To the best of our knowledge, this is a report of the prevalence and characterization of erm(A)-positive C. coli from chicken cecum. A cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increased erythromycin MIC in Campylobacter coli.

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鸡盲肠大肠弯曲杆菌核糖体RNA甲基化酶基因erm(A)及其启动子突变的研究

大环内酯类抗生素通常用于治疗弯曲杆菌病在临床设置和畜牧业。大环内酯耐药弯曲杆菌的出现带来了公共卫生风险。本研究从鸡盲肠样本中鉴定出6株携带erm(A)基因的大肠弯曲杆菌，并对erm(A)的功能和遗传环境进行了分析。对弯曲杆菌分离株进行了药敏试验。采用WGS分析erm(A)阳性菌株的遗传特性。通过克隆和体外诱导耐药实验对erm(A)基因的功能进行了研究。208份鸡盲肠标本共检出大肠杆菌42株（20.2%），其中erm(A)阳性6株。诱导耐药实验显示erm(a)启动子中胞嘧啶插入- 54 bp/ - 55 bp，导致红霉素MIC增加4倍。WGS分析显示，大肠杆菌erm(A)基因与粪肠球菌erm(A)基因在IS1216之间具有高核苷酸序列同源性，与optrA基因共定位。据我们所知，这是一份关于鸡盲肠erm(a)阳性大肠杆菌的流行和特征的报告。大肠弯曲杆菌erm(A)启动子- 54 bp/ - 55 bp的胞嘧啶插入，导致红霉素MIC增加4倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International journal of food microbiology 工程技术-食品科技

CiteScore

10.40

自引率

5.60%

发文量

322

审稿时长

65 days

期刊介绍： The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.