Harnessing miR-16-5p-Loaded Exosomes from Adipose-Derived Stem Cells to Restore Immune Homeostasis in SLE Patients

IF 2.5 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2025-03-06 DOI:10.1016/j.imbio.2025.152885

Yin-hong Zhang , Juan Chen , Wen-fei Mao , Li-xi Yan , Fei Long , Sheng-hao Li , Rui-xian Zhang

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引用次数: 0

Abstract

Background

Systemic Lupus Erythematosus (SLE) is an autoimmune disorder marked by an imbalance between pro-inflammatory Th17 cells and regulatory T cells (Tregs), which contributes to chronic inflammation and multi-organ damage, necessitating novel therapeutic strategies.

Methods

This study investigates the potential of adipose-derived stem cell (ADSC) exosomes to modulate the Th17/Treg balance in SLE patients through the miR-16-5p/LATS1 axis. Flow cytometry, ELISA, and quantitative real-time PCR were utilized to assess immune cell populations and cytokine levels in SLE patients. Additionally, ADSC exosomes were isolated and characterized, and their impact on CD4+ T cells was evaluated using dual-luciferase and Western blot assays.

Results

SLE patients exhibited increased Th17 cells and decreased Tregs, with corresponding changes in cytokine levels. Reduced miR-16-5p expression was noted in CD4+ T cells, correlating positively with Treg proportions. ADSC-derived exosomes were shown to deliver miR-16-5p effectively, targeting and downregulating LATS1 expression. This modulation restored the Th17/Treg balance and adjusted cytokine expression, indicating an immune regulatory effect.

Conclusion

ADSC-derived exosomes, through the miR-16-5p/LATS1 axis, offer a promising therapeutic approach for SLE by restoring immune equilibrium. This study highlights the potential of exosome-based therapies in modulating immune responses, providing a foundation for developing innovative treatments for autoimmune diseases.

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利用来自脂肪来源干细胞的mir -16-5p负载外泌体恢复SLE患者的免疫稳态

系统性红斑狼疮（SLE）是一种自身免疫性疾病，其特征是促炎Th17细胞和调节性T细胞（Tregs）之间的失衡，导致慢性炎症和多器官损伤，需要新的治疗策略。方法本研究探讨了脂肪源性干细胞（ADSC）外泌体通过miR-16-5p/LATS1轴调节SLE患者Th17/Treg平衡的潜力。利用流式细胞术、ELISA和实时荧光定量PCR来评估SLE患者的免疫细胞群和细胞因子水平。此外，我们分离并鉴定了ADSC外泌体，并使用双荧光素酶和Western blot方法评估了它们对CD4+ T细胞的影响。结果ssle患者Th17细胞增多，Tregs细胞减少，细胞因子水平发生相应变化。CD4+ T细胞中miR-16-5p表达降低，与Treg比例呈正相关。adsc衍生的外泌体被证明可以有效地传递miR-16-5p，靶向并下调LATS1的表达。这种调节恢复了Th17/Treg平衡，调节了细胞因子的表达，表明具有免疫调节作用。结论adsc衍生的外泌体通过miR-16-5p/LATS1轴，通过恢复免疫平衡为SLE提供了一种有希望的治疗方法。这项研究强调了外泌体治疗在调节免疫反应方面的潜力，为开发自身免疫性疾病的创新治疗方法提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.