Magnetic MOF-based sensing platform integrated with graphene field-effect transistors for ultrasensitive detection of infectious disease

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-03-06 DOI:10.1016/j.bioelechem.2025.108951

Yushuang Liu , Mingxuan Wang , Guiqi Zhou , Ying Zhang , Wenfeng Hai

引用次数: 0

Abstract

The development of highly sensitive methods for detecting infectious diseases is crucial for preventing disease spread. In this study, a novel sensing platform for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogens was developed by combining a magnetic metal-organic framework (Fe₃O₄@MIL-100) with graphene field-effect transistors (GFET). The Fe₃O₄@MIL-100 magnetic MOF was functionalized with SARS-CoV-2-specific antibodies, enabling highly selective pathogen capture in a phosphate-buffered solution. Following magnetic separation, the captured pathogens were detected using GFETs, with a linear detection range of 1 ag/mL to 10 ng/mL and a detection limit as low as 8.60 ag/mL. Furthermore, the platform has been successfully applied to human serum samples, highlighting its remarkable potential for practical application.

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集成石墨烯场效应晶体管的磁mof传感平台，用于传染病的超灵敏检测

开发高灵敏度的传染病检测方法对于预防疾病传播至关重要。在这项研究中，将磁性金属有机框架（Fe3O4@MIL-100）与石墨烯场效应晶体管（GFET）相结合，开发了一种检测严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）病原体的新型传感平台。Fe3O4@MIL-100磁性MOF用sars - cov -2特异性抗体功能化，能够在磷酸盐缓冲溶液中高度选择性地捕获病原体。磁分离后，利用gfet对捕获的病原体进行检测，线性检测范围为1 ag/mL ~ 10 ng/mL，检出限低至8.60 ag/mL。此外，该平台已成功应用于人血清样本，突出了其显著的实际应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.