Molecular and serological evidence of chikungunya virus among dengue suspected patients in Sri Lanka

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-02-19 DOI:10.1016/j.jiph.2025.102709

Mya Myat Ngwe Tun , Maurine Mumo Mutua , Shingo Inoue , Yuki Takamatsu , Satoshi Kaneko , Takeshi Urano , Rohitha Muthugala , Lakkumar Fernando , Menaka Hapugoda , YINS Gunawardene , Kouichi Morita

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引用次数: 0

Abstract

Background

Chikungunya virus (CHIKV), transmitted by Aedes mosquitoes, causes significant morbidity characterized by acute febrile illness to chronic and permanent disability in some patients. Despite its potential for severe long-term effects, surveillance for CHIKV remains limited, especially in dengue-endemic region like Sri Lanka. To address the gap in surveillance, this study aimed to determine the prevalence of CHIKV among patients suspected of dengue fever during the 2017–2019 DENV outbreak in Sri Lanka.

Methods

Serum samples were collected from 295 patients at Kandy National Hospital and 300 patients at Negombo Hospitals, presenting with dengue-like symptoms such as fever, rash, and arthralgia. We performed quantitative real-time RT-PCR (RT-qPCR) to detect the CHIKV genome and conducted serological tests for anti-CHIKV IgM and IgG antibodies on all samples. Serology-positive samples were further validated with neutralization assays to confirm CHIKV-specific antibodies.

Results

The prevalence of recent CHIKV infection (IgM or RT-qPCR positive) was 2.4 % and 7.0 %, while past CHIKV infection (IgG-positive) was 16.3 % and 12.3 % with neutralizing antibody (NAb) in Kandy and Negombo Hospitals, respectively. All IgG-positive samples exhibited NAb with titers of 10 or higher. The NAb geometric mean titer in the Kandy and Negombo areas does not show a significant difference. In the Kandy area, the majority of CHIKV infections occurred in young adults aged 13–24 years, accounting for 57.1 % of recent infections and 52.1 % of past infections.

Conclusion

CHIKV circulates alongside DENV outbreaks, with a higher prevalence of recent infections in Negombo compared to Kandy, while past infections are more common in Kandy than in Negombo. Neutralization assays confirmed the presence of CHIKV-specific antibodies, emphasizing the need for enhanced surveillance for proper patient care and management. These findings underscore the importance of public health interventions, including surveillance programs and vaccine development, to mitigate the burden of CHIKV in Sri Lanka.

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.