Developed BL-EF to acquire plant growth-promoting functions under salt stress by introducing the ACC deaminase gene

IF 6.1 2区 生物学 Q1 PLANT SCIENCES
Xinyu Jia , Linlin Sun , Junsong Yue , Xiaohu Zhou , Zihe Deng , Xiaochen Liu , Zhansheng Wu
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引用次数: 0

Abstract

The application of plant growth-promoting rhizobacteria (PGPR) is a novel and effective strategy to ameliorate soil salinity and increase agricultural productivity. ACC deaminase (ACCD) in PGPR plays a key role in alleviating salt stress and promoting plant growth. This study aimed to investigate the potential of ACCD-producing strain BL-EF to mitigate salt stress in tomato plants. The ACCD gene was introduced into the non-PGPR Escherichia coli to successfully construct to construct BL-EF and produce catalytically active ACCD. The results showed that strain BL-EF significantly increased the height of tomato plants by 30.94% and 44.63%, under both normal and salt stress conditions, respectively. Strain BL-EF also modulated the photosynthetic pigmentation process in plants, promoting plant growth and increasing tomato tolerance to salt stress. The osmoregulatory system improved and the antioxidant enzyme activities increased to counteract reactive oxygen species-induced activities inoculated with BL-EF compared with those not inoculated with BL-EF. In addition, the inoculation with BL-EF strains increased soil enzyme activities and enhanced nutrients availability in the soil for plants uptake. In conclusion, the inoculation of ACC deaminase-producing strain BL-EF holds immense potential to alleviate salt stress in tomato plants, offering significant benefits to the agricultural sector.

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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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