Preliminary findings of DNA hypermethylation of MDGA1 in idiopathic restless legs syndrome

IF 3.8 2区医学 Q1 CLINICAL NEUROLOGY

Sleep medicine Pub Date : 2025-02-27 DOI:10.1016/j.sleep.2025.02.042

Xiao-Ying Zhu , Xin-Rong He , Yu Wang , Chun-Ni Guo , Hong-Ming Wang , Xuan Li , Xi-Xi Wang , Jing Zhang , Ya Feng , Jing-Tao Feng , Jia-Peng Zhao , Shu-Qin Chen , Yue Zhang , Te Liu , Yun-Cheng Wu

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引用次数: 0

Abstract

Background

Both genetic and environmental factors contribute to the development of restless legs syndrome (RLS). Epigenetic mechanisms might play a vital role in RLS but remain underexplored. MDGA1, involved in synaptic inhibition, has been identified by genome-wide association studies as a potential risk gene for RLS. However, its role and underlying mechanisms in RLS are largely unknown.

Objective

To investigate the relationship between DNA methylation levels in the promotor region of MDGA1 and RLS susceptibility and phenotypes.

Methods

Two independent RLS cohorts (including three large RLS families) and healthy controls (HCs) were recruited. Clinical characteristics were recorded, and DNA methylation levels of CpG islands in the MDGA1 gene from peripheral blood mononuclear cells were measured. Associations between MDGA1 methylation (MDGA1m) and RLS phenotypes (age, sex, and family history) were also analyzed.

Results

A total of 62 idiopathic RLS (iRLS) patients (29 from Cohort 1 and 33 from Cohort 2) and 45 healthy controls (24 from Cohort 1 and 21 from Cohort 2) were included. MDGA1 methylation levels were significantly higher in iRLS patients compared to HCs. Among RLS families, both RLS patients and non-RLS family members showed hypermethylation compared to HCs. Moreover, a positive family history of RLS was associated with an increased risk of MDGA1 hypermethylation.

Conclusion

Our study identified hypermethylation of the MDGA1 gene in the peripheral blood of RLS cases, which may be linked to family history.

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来源期刊

Sleep medicine 医学-临床神经学

CiteScore

8.40

自引率

6.20%

发文量

1060

审稿时长

49 days

期刊介绍： Sleep Medicine aims to be a journal no one involved in clinical sleep medicine can do without. A journal primarily focussing on the human aspects of sleep, integrating the various disciplines that are involved in sleep medicine: neurology, clinical neurophysiology, internal medicine (particularly pulmonology and cardiology), psychology, psychiatry, sleep technology, pediatrics, neurosurgery, otorhinolaryngology, and dentistry. The journal publishes the following types of articles: Reviews (also intended as a way to bridge the gap between basic sleep research and clinical relevance); Original Research Articles; Full-length articles; Brief communications; Controversies; Case reports; Letters to the Editor; Journal search and commentaries; Book reviews; Meeting announcements; Listing of relevant organisations plus web sites.