Poncirin ameliorates alcoholic liver injury by regulating lipid metabolism and inflammatory response in a PPARα dependent manner

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-03-02 DOI:10.1016/j.phymed.2025.156598

Jiawen Huang , Kaili Zhou , Jiayu Li , Zaibin Xu , Xiaoqin Wu , Tingting Chen , Danna Wang , Huafeng Pan , Shuiqing Huang , Tianwen Liu , Zhuohui Luo , Yong Gao

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引用次数: 0

Abstract

Background

Poncirin (PO) is a citrus flavonoid with various of functional effect including cardiac ischemia-reperfusion injury, colitis, cancer, et al. Considering the role of PO in improving inflammation and lipid metabolism, it may have potential therapeutic effects on alcoholic liver injury (ALI), but there are currently no relevant reports.

Purpose

Current study aimed to explore the protective effect of PO on preventing ALI.

Methods

A chronic ethanol-fed mice was used as ALI-mice model and ethanol-induced mouse primary hepatocytes (MPHs) as ALI-cells model. Multiple molecular biology analysis methods are used to evaluate PO's efficacy.

Results

Both in vivo and in vitro, PO improved the inflammatory response and lipid deposition induced by ethanol. According to RNA-seq analysis, Peroxisome proliferator activated receptor alpha (PPARα) had been found as a potential target, followed by the experiment validation using Cellular Thermal Shift Assay (CETSA), Western bolt analysis as well as qPCR analysis. In addition, the protective effect of PO was reduced or disappeared in PPARα^-/- ALI mice, both in vivo and in vitro.

Conclusion

This is the first study to evaluate the role of PO in preventing ALI by targeting lipid metabolism and the inflammatory response by partly targeting the PPARα pathway, providing a fundamental basis for the use of PO as a functional food to alleviate ALI.

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.