Autoimmunity in chronic HDV infection: results from the HDV Describe study cohort

IF 4 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Digestive and Liver Disease Pub Date : 2025-02-01 DOI:10.1016/j.dld.2025.01.094

G.P. Caviglia , E. Dileo , T. Manetta , B. Donati Marello , P. Merlach , A. Loglio , M. Viganò , S. Fagiuoli , P. Colombatto , B. Coco , A. Olivero , G. Troshina , A. Ciancio , D. Angrisani , G.G. Di Costanzo , C.F. Magni , S. Antinori , G. Rizzardini , V. Messina , M. Milella , M. Rizzetto

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引用次数: 0

Abstract

Introduction

Chronic liver disease induced by hepatitis D virus (HDV) infection is associated with autoimmune manifestations.

Aim

The present study investigated the prevalence of autoantibodies (auto-ab) and their association with clinical and virologic features in patients with chronic HDV infection.

Materials and Methods

We studied 494 patients with chronic HDV infection from the HDV Describe study cohort. Non-organ-specific auto-ab (e.g., anti-nuclear auto-ab [ANA], anti-smooth muscle auto-ab [ASMA], anti-mitochondrial auto-ab [AMA], and anti-liver-kidney microsomal auto-ab [LKM]) were tested in diluted serum samples (1:80) by indirect immunofluorescence (IIF) on rat kidney/stomach/liver tissue slides (Euroimmun, Germany). Selected serum samples were analysed by immunoblot (IB) (Euroimmun, Germany) to detect antigen-specific auto-ab.

Results

The prevalence of ANA, ASMA, AMA, and LKM by IIF was 9.9% (n=49), 12.3% (n=61), 2.0% (n=10), and 1.8% (n=9), respectively. Among LKM+ patients, only one patient was LKM-1+ at IB. A high proportion of patients had anti-brush border auto-ab (n=99; 20.0%); 9 patients were anti-parietal cells auto-ab+, 1 was anti-reticulin auto-ab+, and 1 was anti-lysosome auto-ab+. ASMA+ was associated with liver cirrhosis (OR=2.00, 1.08–3.70) and HDV RNA >3 Log IU/mL (OR=1.91, 1.03–3.52), whereas no association was observed between ASMA+ and ALT >40 U/L (OR=1.12, 0.65–1.93). At multivariate analysis, ASMA+ resulted significantly associated with liver cirrhosis (OR=1.89, 1.01–3.51) independently from HDV RNA (OR=1.57, 1.01–2.42) and ALT (OR=1.22, 0.79–1.87).

Conclusion

The assessment of circulating auto-ab by IIF may represent a valuable and inexpensive tool for identifying patients with high HDV replication and advanced liver disease.

This research was supported by Gilead Sciences, Inc (study ID: IN-IT-980-6382).

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来源期刊

Digestive and Liver Disease 医学-胃肠肝病学

CiteScore

6.10

自引率

2.20%

发文量

632

审稿时长

19 days

期刊介绍： Digestive and Liver Disease is an international journal of Gastroenterology and Hepatology. It is the official journal of Italian Association for the Study of the Liver (AISF); Italian Association for the Study of the Pancreas (AISP); Italian Association for Digestive Endoscopy (SIED); Italian Association for Hospital Gastroenterologists and Digestive Endoscopists (AIGO); Italian Society of Gastroenterology (SIGE); Italian Society of Pediatric Gastroenterology and Hepatology (SIGENP) and Italian Group for the Study of Inflammatory Bowel Disease (IG-IBD). Digestive and Liver Disease publishes papers on basic and clinical research in the field of gastroenterology and hepatology. Contributions consist of: Original Papers Correspondence to the Editor Editorials, Reviews and Special Articles Progress Reports Image of the Month Congress Proceedings Symposia and Mini-symposia.