Analysis of the two-component system gene family and the positive role of CsRR5 in cold stress response in tea plants

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-04 DOI:10.1016/j.plaphy.2025.109739

Xiaobin Dong , Changqing Ding , Xuening Zhang , Lei Lei , Yao Chen , Qianyuan Fu , Ying Yang , Yuwan Hao , Meng Ye , Jianming Zeng , Xinchao Wang , Wenjun Qian , Jianyan Huang

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引用次数: 0

Abstract

The two-component system (TCS), a ubiquitous signaling network consisting of histidine kinases (HKs), phosphotransfers (HPs), and response regulator proteins (RRs), participates in various functions, including responses to abiotic stresses. However, a comprehensive identification of TCS genes in tea plants is still lacking. Here, we identified 60 CsTCS members in tea plants, including 23 HKs, 10 HPs, and 27 RRs. The analysis of promoter cis-regulatory elements indicated that CsTCS genes are involved in phytohormone signaling, stress responses, and growth and development. The expression of CsETR1, CsETR2b, CsERS1b, and CsEIN4b from the HK subfamily was down-regulated by ethylene, whereas CsHK2b and CsHK3a were down-regulated by cytokinin. Conversely, CsHK4a and CsHK4b were up-regulated by cytokinin. CsTCS genes were widely expressed in various tissues, with the majority associated with multiple stresses. Furthermore, we demonstrated that suppressing the type-A response regulator CsRR5 in tea plants reduced cold tolerance and the expression of CBF-COR pathway genes, indicating that CsRR5 positively regulates the cold stress response through the CBF pathway. Therefore, our study establishes a connection between the two-component system and its downstream regulation in tea plant cold stress response.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.