Reference genes assessment and selection for expression analyses by qRT-PCR in Euwallacea interjectus

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-07 DOI:10.1016/j.genrep.2025.102188

Nan Jiang, Jialin Liu, Shengchang Lai, Yang Zhou, Dejun Hao, Lulu Dai

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引用次数: 0

Abstract

Euallacea interjectus (Coleoptera, Curculionidae: Scolytinae) is an emerging pest in poplar plantations, capable of parasitizing living trees and cultivating Ambrosia Fusarium Clade (AFC) fungi as a nutritional source. Host trees infested by E. interjectus experience severe decline and wilting symptoms. Despite the considerable impact of E. interjectus, no reference genes for gene expression analysis have been documented for this species. This study identified 12 reference genes from the E. interjectus transcriptome. Their expression genes were evaluated using RT-qPCR and assessed through five methodologies: CT value calculation, BestKeeper, NormFinder, RefFinder, and geNorm. Among the identified genes, EIS6K1, EITOR, EIRPL13, and EIRPL18 exhibited the highest stability in female specimens, while EIPRS3a and EIRPS18 were the most stable in male specimens. These findings confirm the reliability of the selected algorithms and provide a strong foundation for future research in molecular biology and pest control strategies.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.