Identifying the role of Casparian strip membrane proteins in rice under salinity stress

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-07 DOI:10.1016/j.genrep.2025.102192

Suraj Patil, Shrushti Joshi, Vinay Kumar

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引用次数: 0

Abstract

Casparian strip (CS) is a specialized structure in endodermal cell layer of plant roots and is critical for regulating hydro-mineral transport and thus plant growth and stress responses. CS are synthesized and stabilized by a specialized class of proteins called CS-membrane-domain-proteins, the CS membrane proteins (CASPs) (CASPs). CASPs have been identified in various crops; however, such studies on Indica rice cultivars remain limited. This study was conducted to identify and characterize putative CASPs in Indica rice varieties, and their influence on expression patterns of key genes under salinity stress. The genome-wide screening revealed five putative orthologs in the Indica genome, three of them were confirmed as stable and functional CASPs through Eggnogg, Protparam, gene structure and domain architecture analysis. Cis-regulatory element analysis showed abscisic acid-responsive element (ABRE) and anaerobic responsive element (ARE) promoter within the promoter region, indicating their roles in regulating plant hormones, stress-responses, and influencing developmental processes. Subcellular localization confirmed their presence on plasma membrane. Three-dimensional structures prediction showed that all CASPs have four transmembrane architectures. To analyze responses under salinity stress, two Indica rice varieties differing in salinity tolerance abilities were studied for their responses to salinity stress including reactive oxygen species generation, lipid peroxidation, and responsive antioxidant enzyme activities. Varietal and stress-responsive differential enzyme activity was seen particularly for peroxidases, which are known to be involved in CS formation. Histochemical visualization for the development of CS using propidium iodide diffusion confirmed the varietal difference under salinity stress. Differential qRT-PCR-based gene expression of identified genes further confirmed the role of CS under salinity stress.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.