Genetic insights and phylogenetic relationships of Momordica balsamina using DNA barcoding: An accurate tool for species identification

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-07 DOI:10.1016/j.genrep.2025.102186

Iqra Aman , Quratulain Maqsood , Tahir Mehmood , Aleena Sumrin , Rehman Shahzad

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引用次数: 0

Abstract

The Momordica balsamina, a member of Cucurbitaceae family is a plant species of immense botanical, phytochemicals, pharmacological, and medicinal value. Accurate identification is crucial in order to extract valuable bioactive compounds which is important for ensuring the quality and safety of herbal medicines. While traditional taxonomic methods aid in species identification but an innovative and authentic method for identifying species is DNA barcoding, which is based on the nucleotide diversity of conserved sequences. Plant DNA barcoding systems are more difficult to build and refine because of the enormous genetic variation among species. To ensure a more reliable approach, plant scientists should focus on conserved nuclear-transcribed regions to analyze genetic diversity, species differentiation, and phylogeny. In this study, we amplified and sequenced the rbcL, matK, and ITS regions of Momordica balsamina. Furthermore, there Phylogenetic tree analysis, bootstrap percentage, sequence homology and failed amplification of matK were discussed. M. balsamina species were authenticated by using ITS primer (internal transcribed spacer). Through this intended research, we aim to genetically identify and characterize this medicinally important species through barcoding.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.