A Novel Near-Infrared AIE Probe for Sensitive Imaging of Lipid Droplet and Dual-Parameter Cancer Diagnosis

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-03-08 DOI:10.1016/j.aca.2025.343916

Cong Liu, Shan He, Xiao-Feng Guo, Hong Wang

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引用次数: 0

Abstract

Background

Lipid droplets (LDs) are vital intracellular organelles for lipid storage, closely associated with various metabolic disorders and cancers. Fluorescence imaging offers a powerful, non-invasive approach to study LDs in real time, but many existing probes suffer from non-specific staining and aggregation-caused quenching (ACQ), compromising their imaging specificity and contrast.

Result

In this study, we synthesized a novel LD fluorescent probe TPC-AN that takes advantage of near-infrared emission, large Stokes shift, high lipophilicity, polarity response and aggregation-induced emission (AIE) characteristics. TPC-AN effectively addresses issues of non-specific staining and ACQ commonly observed with traditional probes, enabling highly specific and high-contrast imaging of LDs. Utilizing TPC-AN, imaging of LDs in several kinds of cells was performed, and discrimination of cancerous and normal cells was achieved using dual-parameter through differences in LD fluorescence area and intensity.

Significance

This work provides a promising tool for studying LDs in diseases and offers a reliable method for cancer diagnosis, with excellent LD-specificity, low cytotoxicity, and dual-parameter imaging capabilities.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.