Mitochondrial genome and transcription of Shiraia-like species reveal evolutionary aspects in protein-coding genes.

IF 5.2 1区 生物学 Q1 MYCOLOGY
Ima Fungus Pub Date : 2025-02-20 eCollection Date: 2025-01-01 DOI:10.3897/imafungus.16.138572
Xiao-Ye Shen, Xue-Ting Cao, Xiao-Bo Huang, Lan Zhuo, Hui-Meng Yang, Li Fan, Cheng-Lin Hou
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引用次数: 0

Abstract

Shiraia-related species are well-known bambusicolous fungi in Dothideomycetes class, with high value in traditional medicine for producing hypocrellin, as an anticipated photosensitiser. The complete mitogenomes of hypocrellin-producing Pseudoshiraiaconidialis strains were analysed in the present study, with functional gene variations through comparative genomics and transcriptomics. Five strains (ZZZ816, CNUCC1353PR, JAP103846, CNUCC C72, CNUCC C151) were sequenced, which indicated similar genome characteristics. Two of them possess an extra atp6 gene, and the associated variable fragment "HSP1-HSP2-atp6_2" correlates closely with hypocrellin production capacity. Therefore, these five strains were divided into three groups: ZZZ816 and CNUCC1353PR possessing high production efficiency, CNUCC C72 and JAP103846 with low yield and CNUCC C151 as a transition type. The gene expression changes were screened under various conditions. ZZZ816-related species showed significant changes in mitochondrial genes, especially HSP1, HSP2 and atp6_2, linked closely to hypocrellin synthesis and stress response; rps3 expression also consistently correlated with hypocrellin production. JAP103846 group showed a stable expression pattern divergently, except for rps3 suppression by blue light. These findings would provide new insights into secondary metabolite regulation and ROS resistance. Above all, this study conducted the comprehensive analysis of Shiraia-like fungi mitogenomes and functional gene expression, which can update the understanding of fungal evolution and potential for improved hypocrellin production.

Mitochondrial白井样物种的基因组和转录揭示了蛋白质编码基因的进化方面。
白叶菌属的近缘种是众所周知的竹类真菌,在传统医学中具有很高的价值,可生产下皮草素,是一种值得期待的光敏剂。本研究通过比较基因组学和转录组学分析了产生hypocrellin的假shiraiaconidialis菌株的完整有丝分裂基因组,并分析了功能基因变异。5株菌株(ZZZ816、CNUCC 1353pr、JAP103846、CNUCC C72、CNUCC C151)测序结果表明,它们具有相似的基因组特征。其中两种具有一个额外的atp6基因,相关的可变片段“HSP1-HSP2-atp6_2”与hypocrellin的生产能力密切相关。因此,将这5个菌株分为生产效率较高的ZZZ816和CNUCC1353PR,产量较低的CNUCC C72和JAP103846,过渡型的CNUCC C151。筛选不同条件下的基因表达变化。zzz816相关种的线粒体基因发生了显著变化,尤其是与下丘脑素合成和应激反应密切相关的HSP1、HSP2和atp6_2;Rps3的表达也与hypocrellin的产生一致相关。除蓝光抑制rps3外,JAP103846组表现出稳定的发散性表达模式。这些发现将为研究次生代谢物调控和ROS抗性提供新的见解。综上所述,本研究对白井样真菌的有丝分裂基因组和功能基因表达进行了全面的分析,可以更新对真菌进化的认识,并有可能提高hypocrellin的产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Ima Fungus
Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍: The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue
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