Assembly and comparative analysis of the complete mitochondrial genome of Daedaleopsissinensis (Polyporaceae, Basidiomycota), contributing to understanding fungal evolution and ecological functions.

Abstract

Daedaleopsissinensis is a crucial wood-decaying fungus with significant lignocellulose-degrading ability, which plays a vital role in the material cycle and energy flow of forest ecosystems. However, the mitochondrial genome of D.sinensis has not yet been revealed. In the present study, the complete mitochondrial genome of D.sinensis was assembled and compared with related species. The mitochondrial genome spans 69,155 bp and has a GC content of 25.0%. It comprises 15 protein-coding genes (PCGs), 26 transfer RNA genes, two ribosomal RNA genes and one DNA polymerase gene (dpo). Herein, we characterised and analysed the codon preferences, variation and evolution of PCGs, repeats, intron dynamics, as well as RNA editing events in the D.sinensis mitochondrial genome. Further, a phylogenetic analysis of D.sinensis and the other 86 Basidiomycota species was performed using mitochondrial genome data. The results revealed that four species, D.confragosa, D.sinensis, D.nitida and Fomesfomentarius, were grouped in a closely-related cluster with high support values, indicating that a close phylogenetic relationship existed between Daedaleopsis and Fomes. This study reported on the initial assembly and annotation of the mitochondrial genome of D.sinensis, which greatly improved the knowledge of the fungus. These results contribute to the limited understanding of the mitochondrial repository of wood-decaying fungi, thereby laying the foundation for subsequent research on fungal evolution and ecological functions.