A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-03-06 DOI:10.1186/s12985-025-02671-4

Shunchang Fan, Minyi Zhang, Yucheng Li, Jingli Tian, Juxian Xian, Qing Chen

{"title":"A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.","authors":"Shunchang Fan, Minyi Zhang, Yucheng Li, Jingli Tian, Juxian Xian, Qing Chen","doi":"10.1186/s12985-025-02671-4","DOIUrl":null,"url":null,"abstract":"Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples. The approach demonstrated high sensitivity and specificity, with detection limits of 1 × 102 copies/µL for kobuvirus, parechovirus B, and rosavirus B, and 50 copies/µL for hunnivirus. Evaluation using 149 clinical samples showed strong concordance with conventional PCR methods.Conclusions: This work developed an effective multiplex qPCR method for the simultaneous detection of emerging picornaviruses particularly in rodents, including kobuvirus, parechovirus B, rosavirus B, and hunnivirus. Our findings contribute valuable insights into the monitoring and prevention of zoonotic diseases associated with these pathogens.","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"61"},"PeriodicalIF":4.0000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883910/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12985-025-02671-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.

Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples. The approach demonstrated high sensitivity and specificity, with detection limits of 1 × 10² copies/µL for kobuvirus, parechovirus B, and rosavirus B, and 50 copies/µL for hunnivirus. Evaluation using 149 clinical samples showed strong concordance with conventional PCR methods.

Conclusions: This work developed an effective multiplex qPCR method for the simultaneous detection of emerging picornaviruses particularly in rodents, including kobuvirus, parechovirus B, rosavirus B, and hunnivirus. Our findings contribute valuable insights into the monitoring and prevention of zoonotic diseases associated with these pathogens.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.