Dasatinib and Quercetin Mitigate Age-Related Alveolar Bone Inflammaging and Neutrophil Infiltration.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-03-06 DOI:10.1111/odi.15291

Jinru Tang, Jingya Li, Zeyu Hou, Rong He, Bingzhi Li, Jiajing Gong, Yuhang Xie, Wanrong Meng, Yunkun Liu, Takehito Ouchi, Longjiang Li, Bo Li

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引用次数: 0

Abstract

Objective: Age-related alveolar bone resorption poses a major dental health challenge, yet its mechanisms and treatments are poorly understood. This study investigates the impact of dasatinib and quercetin (D + Q) treatment on senescent cells (SnCs), senescence-associated secretory phenotype (SASP), and neutrophil infiltration in aged alveolar bone, aiming to develop new strategies for combating age-related bone resorption.

Methods: C57BL/6 mice (2 and 18 months) were used to examine alveolar bone resorption, inflammaging, and neutrophil infiltration. Aged mice received D + Q treatment to assess therapeutic effects. Key measurements included cementoenamel junction to the alveolar bone crest (CEJ-ABC) distance, periodontal ligament (PDL) thickness, osteometabolism markers, SnCs accumulation, SASP expression, and neutrophil infiltration.

Results: Aged alveolar bone showed increased CEJ-ABC distance, atrophied periodontal ligament, and unbalanced osteometabolism, along with elevated SnCs, SASP, and neutrophils compared to young controls. D + Q treatment improved these conditions by reducing CEJ-ABC distance, enhancing periodontal ligament health, and boosting bone metabolism. It also lowered the expression of SnCs, SASP, and neutrophil markers.

Conclusion: D + Q treatment effectively mitigates alveolar bone aging by clearing SnCs, lowering SASP levels, and reducing neutrophil aggregation, presenting a novel approach for age-related bone resorption.

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达沙替尼和槲皮素减轻与年龄相关的牙槽骨炎症和中性粒细胞浸润。

目的：年龄相关的牙槽骨吸收是牙齿健康的主要挑战，但其机制和治疗尚不清楚。本研究探讨了达沙替尼和槲皮素（D + Q）治疗对衰老牙槽骨中衰老细胞（SnCs）、衰老相关分泌表型（SASP）和中性粒细胞浸润的影响，旨在研究对抗衰老相关骨吸收的新策略。方法：采用C57BL/6小鼠（2月龄和18月龄）观察牙槽骨吸收、炎症和中性粒细胞浸润情况。老龄小鼠接受D + Q治疗，观察其治疗效果。主要测量指标包括牙骨质连接到牙槽骨嵴（CEJ-ABC）的距离、牙周韧带（PDL）的厚度、骨代谢标志物、SnCs积累、SASP表达和中性粒细胞浸润。结果：与年轻对照组相比，老年牙槽骨表现为CEJ-ABC距离增加，牙周韧带萎缩，骨代谢不平衡，SnCs， SASP和中性粒细胞升高。D + Q治疗通过减少CEJ-ABC距离、改善牙周韧带健康和促进骨代谢来改善这些疾病。它还降低了SnCs、SASP和中性粒细胞标志物的表达。结论：D + Q治疗通过清除SnCs、降低SASP水平和减少中性粒细胞聚集有效减缓牙槽骨老化，为年龄相关骨吸收提供了一种新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.