Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors.

IF 10.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xingyi Wang, Hanyi Fang, Wenzhu Hu, Yuan Feng, Zhangyongxue Zhou, Mengyan Hu, Dawei Jiang, Yongxue Zhang, Xiaoli Lan
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引用次数: 0

Abstract

Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic oxygen-delivering nanoparticles (CmPF) are engineered for homologous targeting and hypoxia alleviation within the tumor environment. CmPF targets the tumor microenvironment and delivers oxygen to reduce hypoxia, thereby promoting immune cell activity at the tumor site. In addition, granzyme B-targeted positron emission tomography (PET) imaging is employed to monitor immune cell activity changes in response to immunotherapy efficacy in vivo. The combination of CmPF with carboplatin and PD-1 inhibitors significantly suppresses tumor growth by 2.4-fold, exhibiting the potential of CmPF to enhance the efficacy of immunotherapy. Immunohistochemistry further confirms increased expression of key immune markers, highlighting the reprogramming of the tumor microenvironment. This study demonstrates that hypoxia alleviation enhances tumor immunotherapy efficacy and introduces a non-invasive PET imaging method for dynamic, real-time assessment of therapeutic response.

通过颗粒酶B PET成像监测,氧递送纳米颗粒增强了恶性肿瘤的免疫治疗效果。
有限的治疗反应和不充分的监测方法阻碍了免疫治疗的成功。在低氧肿瘤微环境中募集和激活免疫细胞是逆转免疫抑制、提高免疫治疗效果的关键。在这项研究中,仿生氧递送纳米颗粒(CmPF)被设计用于肿瘤环境中的同源靶向和缺氧缓解。CmPF以肿瘤微环境为靶点,输送氧气减少缺氧,从而促进肿瘤部位免疫细胞的活性。此外,采用颗粒酶b靶向正电子发射断层扫描(PET)成像,监测体内免疫细胞活性变化对免疫治疗效果的反应。CmPF联合卡铂、PD-1抑制剂可显著抑制肿瘤生长2.4倍,显示了CmPF增强免疫治疗疗效的潜力。免疫组织化学进一步证实了关键免疫标志物的表达增加,强调了肿瘤微环境的重编程。本研究表明,缺氧缓解可提高肿瘤免疫治疗效果,并引入了一种无创PET成像方法,用于动态、实时评估治疗反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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