Limibacterium fermenti gen. nov., sp. nov., a novel member of the family Dysgonomonadaceae isolated from the Chinese strong aroma-type Baijiu fermentation system.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-03-01 DOI:10.1099/ijsem.0.006702

Yang Gu, Huilin Wang, Xia Zhang, Zhiyun Peng, Jian Su, Dong Zhao, Jia Zheng

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引用次数: 0

Abstract

An anaerobic isolate, designated me31^T, was isolated from pit mud in Yibin, Sichuan Province, PR China. Phylogenetic results based on 16S rRNA gene sequence showed that strain me31^T belongs to the family Dysgonomonadaceae, and the most closely related isolated relatives were Seramator thermalis SYSU GA16112^T (93.65%) and Proteiniphilum propionicum JNU-WLY501^T (93.22%). The DNA G+C content was 44.26 mol%. The ANI and AAI values between strain me31^T and the closely related strains were 69.25-71.18% and 69.59-71.15%, respectively. Cells of strain me31^T were Gram-stain-negative, rod-shaped and non-motile. Growth of strain me31^T was observed at 25-37 °C, pH 6.0-8.0 and a salt tolerance range of 0-1.0% (w/v). The predominant respiratory quinone was MK-9. The major fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and C_{17 : 0} 2OH. The polar lipids of strain me31^T were found to consist of phosphatidylethanolamine, three unidentified phospholipids, three unidentified phosphoglycolipids, one unidentified phosphoglycolipid, one unidentified lipid, two unidentified glycolipids and one aminophosphoglycolipid. According to the results of morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic analysis, strain me31^T represents a novel species of a novel genus of the family Dysgonomonadaceae, for which the name Limibacterium fermenti gen. nov., sp. nov. is proposed. The type strain is me31^T (=GDMCC 1.4237^T=KCTC 25756^T=WMCC 10035^T).

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.