24 h severe fluid restriction increases a biomarker of renal injury in healthy males.

IF 2.8 3区医学 Q2 PHYSIOLOGY

European Journal of Applied Physiology Pub Date : 2025-03-07 DOI:10.1007/s00421-025-05749-7

Loris A Juett, Mark P Funnell, Harriet A Carroll, Lewis J James, Stephen A Mears

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引用次数: 0

Abstract

Purpose: Exercise-induced hypohydration exacerbates biomarkers of renal injury, but studies isolating the effects of hypohydration without exercise have produced mixed findings. This study investigated the effects of 24-h severe fluid restriction on biomarkers of renal injury and glucose tolerance.

Methods: Fifteen males (age: 27 ± 5 y; BMI: 24.1 ± 3.8 kg/m²) completed two randomised trials, involving consuming either 40 mL/kg body mass water to maintain euhydration (EU) or severe fluid restriction via limiting water consumption to 100 mL (HYP). A standardised dry food diet was consumed in both trials (~ 300 g water). At baseline and 24 h post-baseline, nude body mass, and blood and urine samples (additional urine sample at 12 h) were collected. An oral glucose tolerance test was conducted after 24-h post-baseline measurements (n = 12).

Results: At 24 h, body mass loss (HYP: - 1.52 ± 0.34%, EU: - 0.24 ± 0.40%), plasma volume loss, serum, and urine osmolality were greater in HYP than EU (P ≤ 0.004). Osmolality-corrected urinary kidney injury molecule-1 (uKIM-1) concentrations were greater in HYP at 12 (HYP: 1.097 ± 0.587 ng/mOsm, EU: 0.570 ± 0.408 ng/mOsm; P < 0.001) and 24-h (HYP: 1.932 ± 1.173 ng/mOsm, EU: 1.599 ± 1.012 ng/mOsm; P = 0.01). There was no trial-by-time interactions for osmolality-corrected urinary neutrophil gelatinase-associated lipocalin concentrations (P = 0.781) or plasma glucose (P = 0.550) and insulin (P = 0.193) concentrations.

Conclusion: Hypohydration produced by 24-h fluid restriction increased proximal tubular injury but did not affect glucose tolerance.

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24小时严重饮水限制会增加健康男性肾损伤的生物标志物。

目的：运动引起的缺水会加剧肾损伤的生物标志物，但分离不运动的缺水影响的研究产生了不同的结果。本研究探讨了24小时严重液体限制对肾损伤生物标志物和葡萄糖耐量的影响。方法：男性15例，年龄27±5岁；BMI: 24.1±3.8 kg/m2)完成了两项随机试验，包括消耗40 mL/kg体重的水来维持脱水（EU）或严格限制液体摄入，将水的摄入量限制在100 mL （HYP）。两个试验均采用标准化干粮饮食（约300克水）。在基线和基线后24小时，收集裸体质量、血液和尿液样本（12小时额外的尿液样本）。基线后24小时测量后进行口服葡萄糖耐量试验（n = 12）。结果：24 h时，HYP组体质量损失（HYP: - 1.52±0.34%,EU: - 0.24±0.40%）、血浆体积损失、血清和尿渗透压均大于EU组（P≤0.004）。经渗透压校正的尿肾损伤分子-1 （uKIM-1）浓度在HYP为12时较高(HYP: 1.097±0.587 ng/mOsm, EU: 0.570±0.408 ng/mOsm；结论：限液24 h引起的低水合作用增加了近端肾小管损伤，但不影响糖耐量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Applied Physiology 医学-生理学

CiteScore

6.00

自引率

6.70%

发文量

227

审稿时长

3 months

期刊介绍： The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.