Association of Conjunctival Cell HLA-DR Expression with the Severity of Signs and Symptoms of Dry Eye Disease at Baseline in the NORTHERN LIGHTS Phase 2 Trial.

IF 1.7 4区 医学 Q3 OPHTHALMOLOGY
Eric J Kuklinski, Neeta S Roy, Morten Præstegaard, Yi Wei, Penny A Asbell
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引用次数: 0

Abstract

Purpose: To assess the association of HLA-DR on ocular surface conjunctival cells with the severity of signs and symptoms of dry eye disease (DED) in patients enrolled in the NORTHERN LIGHTS phase 2 trial.

Methods: Patients with moderate to severe DED were recruited from 10 different countries in Europe. Symptoms of DED were assessed using the visual analog scale (VAS) global discomfort score, and signs via corneal fluorescein staining (CFS), lissamine green staining, Schirmer's test, and tear osmolarity. HLA-DR expression was evaluated via flow cytometry from impression cytology of conjunctival cells using the EyePrim™ device sampled from 205 DED patients.

Results: HLA-DR expression was detected in a mean of 3.4% of cells at baseline. HLA-DR expression was significantly associated with corneal staining severity at baseline (p < 0.01). HLA-DR expression was not significantly associated with DED symptoms (VAS global discomfort score), nor lissamine green staining, Schirmer's test, tear osmolarity, or Sjogren's syndrome.

Conclusion: HLA-DR was present in a wide range of percentages among DED patients indicating the heterogeneous nature of DED. HLA-DR was significantly associated with corneal fluorescein staining suggesting a correlation of the biomarker with ocular surface damage.

在北极光2期试验中结膜细胞HLA-DR表达与基线干眼病体征和症状严重程度的关联
目的:评估参加NORTHERN LIGHTS 2期试验的患者眼表结膜细胞HLA-DR与干眼病(DED)体征和症状严重程度的关系。方法:从欧洲10个不同国家招募中重度DED患者。采用视觉模拟量表(VAS)整体不适评分评估DED的症状,并通过角膜荧光素染色(CFS)、丽丝胺绿染色、Schirmer试验和泪液渗透压评估症状。使用EyePrim™设备对205例DED患者的结膜细胞进行印迹细胞学检测,通过流式细胞术评估HLA-DR的表达。结果:基线时平均3.4%的细胞中检测到HLA-DR表达。在基线时,HLA-DR表达与角膜染色严重程度显著相关(p结论:HLA-DR在DED患者中存在的百分比范围很广,表明DED的异质性。HLA-DR与角膜荧光素染色显著相关,提示该生物标志物与眼表损伤相关。
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来源期刊
Current Eye Research
Current Eye Research 医学-眼科学
CiteScore
4.60
自引率
0.00%
发文量
163
审稿时长
12 months
期刊介绍: The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.
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