Yu Chen, Hong Chen, Zhongyan Han, Yiqiang Cui, Chenghao Situ, Yaling Qi, Qing Cheng, Yan Li
{"title":"PRKCQ Is Dispensable for Spermatogenesis in Mice","authors":"Yu Chen, Hong Chen, Zhongyan Han, Yiqiang Cui, Chenghao Situ, Yaling Qi, Qing Cheng, Yan Li","doi":"10.1002/cbin.70014","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Protein kinase C (PKC) family is evolutionally conserved and involved in various signaling cascades in all cells. Of the family, PRKCQ is dominatingly expressed in testis, however, its molecular functionality in spermatogenesis and male fertility remains unclear. To evaluate the role of PRKCQ in spermatogenesis, <i>Prkcq</i> knockout mice were generated using CRISPR/Cas9 system. Histological and immunofluorescence assays by different markers were employed to assess the testicular cells variation. Sperm parameters were analyzed by computer-assisted sperm analyzer. qPCR assay was used to examine the expression levels of other PKC family genes. We found that PRKCQ was conserved throughout evolution and highly expressed in testis. <i>Prkcq</i><sup>−/−</sup> mice were successfully generated and developed viably. Normal fertility was observed in <i>Prkcq</i><sup>−/−</sup> males. <i>Prkcq</i><sup>−/−</sup> mice exhibited no defects in spermatogenic cells and mature sperm were full in epididymis. Furthermore, there were no differences in sperm motility and progressive motility between <i>Prkcq</i><sup>−/−</sup> males and controls. Our findings report a detailed phenotypic analysis of <i>Prkcq</i><sup>−/−</sup> males and indicate that PRKCQ is not required for spermatogenesis in male mice, which can provide basic information for other researchers.</p></div>","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":"49 5","pages":"522-533"},"PeriodicalIF":3.3000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biology International","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbin.70014","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Protein kinase C (PKC) family is evolutionally conserved and involved in various signaling cascades in all cells. Of the family, PRKCQ is dominatingly expressed in testis, however, its molecular functionality in spermatogenesis and male fertility remains unclear. To evaluate the role of PRKCQ in spermatogenesis, Prkcq knockout mice were generated using CRISPR/Cas9 system. Histological and immunofluorescence assays by different markers were employed to assess the testicular cells variation. Sperm parameters were analyzed by computer-assisted sperm analyzer. qPCR assay was used to examine the expression levels of other PKC family genes. We found that PRKCQ was conserved throughout evolution and highly expressed in testis. Prkcq−/− mice were successfully generated and developed viably. Normal fertility was observed in Prkcq−/− males. Prkcq−/− mice exhibited no defects in spermatogenic cells and mature sperm were full in epididymis. Furthermore, there were no differences in sperm motility and progressive motility between Prkcq−/− males and controls. Our findings report a detailed phenotypic analysis of Prkcq−/− males and indicate that PRKCQ is not required for spermatogenesis in male mice, which can provide basic information for other researchers.
期刊介绍：
Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect.
These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.
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