Influence of Physical Activity and Nutritional Limitation on Amino Acid, Fatty Acid Metabolism, and Biochemical Responses in Juvenile Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1792)

IF 1.9 4区 农林科学 Q2 FISHERIES
Gökhan Tunçelli, Onur Ertik, İdil Can Tunçelli, Bertan Boran Bayrak, Özkan Özden, Refiye Yanardag, Devrim Memiş
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Abstract

This study investigates the synergistic effects of swimming activity and dietary restriction on the metabolic and nutritional characteristics of muscle tissue in juvenile rainbow trout (Oncorhynchus mykiss, Walbaum, 1792). During a 6-week study, four groups of juvenile rainbow trout, each starting with an average weight of 26.54 ± 0.36 g, were analyzed: the first group was allowed to feed freely in static water (SW group), the second experienced a dietary limitation (25% feed restriction) (LF group), the third was required to swim at a speed of one body length per second (SE group), and the fourth group faced a combination of dietary restriction (25% feed restriction) and enforced swimming activity (SELF group). Swimming activity was implemented using a water flow rate of one body length per second (1 BL s−1), ensuring a standardized exercise intensity. Comprehensive analysis revealed significant alterations in biochemical parameters, amino acid composition, and fatty acid profiles in rainbow trout muscle tissue. The results indicate a decrease in histidine levels (p  < 0.05) with the combined effect of both swimming and feeding restrictions. Additionally, cysteine and semi-essential amino acids (EAAs) showed a decrease (p  < 0.05) solely due to the influence of swimming. As for fatty acid outcomes, linolenic acid exhibited a reduction with the combined impact of both swimming and feeding restrictions (p  < 0.05), while margaric acid significantly decreased (p  < 0.05) only with the influence of swimming. Crucial shifts in antioxidant defense mechanisms, including glutathione (GSH) and lipid peroxidation (LPO) levels, were identified, highlighting the roles of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) as biochemical parameters. Detailed examination further clarified modifications in glucose-6-phosphate dehydrogenase (G6PD) activities, reactive oxygen species (ROS) levels, and lactate dehydrogenase (LDH) activity, serving as pivotal indicators of oxidative stress and tissue damage. These findings contribute to a holistic understanding of nutritional dynamics within rainbow trout muscle tissue, offering insights crucial for optimizing fish health and productivity in aquaculture.

Abstract Image

运动和营养限制对虹鳟鱼幼鱼氨基酸、脂肪酸代谢和生化反应的影响(Oncorhynchus mykiss, Walbaum, 1792)
本研究探讨了游泳活动和饮食限制对虹鳟鱼幼鱼肌肉组织代谢和营养特性的协同效应(Oncorhynchus mykiss, Walbaum, 1792)。在六周的研究中,少年虹鳟鱼的四组,每个从平均26.54±0.36克的重量进行了分析:第一组被允许自由饲料在静态水(西南组),第二个经验丰富的饮食限制(限制饲料25%)(低频组),第三次是需要游泳的速度每秒一个身体长度(SE组),和第四组面临的饮食限制(限制饲料25%)和强迫游泳活动(自我组)。游泳活动采用一个体长每秒(1 BL s−1)的水流速率,确保标准化的运动强度。综合分析显示虹鳟鱼肌肉组织的生化参数、氨基酸组成和脂肪酸谱发生了显著变化。结果显示组氨酸水平下降(p <;0.05),游泳和摄食限制共同作用。此外,半胱氨酸和半必需氨基酸(EAAs)呈下降趋势(p <;0.05),完全是由于游泳的影响。至于脂肪酸的结果,亚麻酸在游泳和摄食限制的综合影响下表现出减少(p <;0.05),而人造黄油酸显著降低(p <;0.05),仅受游泳的影响。研究确定了抗氧化防御机制的关键变化,包括谷胱甘肽(GSH)和脂质过氧化(LPO)水平,并强调了过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)作为生化参数的作用。详细的研究进一步阐明了葡萄糖-6-磷酸脱氢酶(G6PD)活性、活性氧(ROS)水平和乳酸脱氢酶(LDH)活性的变化,这些变化是氧化应激和组织损伤的关键指标。这些发现有助于全面了解虹鳟鱼肌肉组织内的营养动态,为优化水产养殖中的鱼类健康和生产力提供至关重要的见解。
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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