Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) infantum sialic acids enhance macrophage infection

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Biology International Pub Date : 2025-01-07 DOI:10.1002/cbin.12269

Tainá Cavalcante, Antônio Moreira Marques, Mariana Medina Medeiros, Tania Carolina Reis, Daniel Quina, Bruna Cunha de Alencar, Giuseppe Palmisano, Beatriz Simonsen Stolf

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引用次数: 0

Abstract

Leishmaniases affect millions of people around the world, caused by Leishmania parasites. Leishmania are transmitted by female sandflies from Phlebotominae subfamily during their blood meals. In mammals, promastigotes are phagocytosed mainly by macrophages, differentiate into amastigotes and multiply. For entry and survival in macrophages, Leishmania uses virulence factors such as surface glycoconjugates. Sialic acids (Sias) are found in terminal portions of glycoconjugates and play important roles in human pathogens. The importance of Sias was explored only in L. (L.) donovani, associated with visceral leishmaniasis in Africa, Asia and Europe. Thus, the aim of this study was to characterize Sias of Leishmania (L.) amazonensis and Leishmania (L.) infantum, related to cutaneous and visceral leishmaniasis in South America, respectively. For that, we analyzed by HPLC-FLD the Sias of promastigotes of L. (L.) amazonensis LV79 and two L. (L.) infantum strains, and of L. (L.) amazonensis axenic amastigotes and amastigotes from paw lesions of infected mice. To evaluate Sias importance in promastigotes, we treated stationary phase parasites with sialidase and infected murine and human macrophages. We detected N-Acetylneuraminic Acid in promastigotes of all strains, with greater abundance in L. (L.) infantum. We identified N-Acetylneuraminic Acid and N-Glycolylneuraminic acid in amastigotes recovered from paw lesion, but only N-Acetylneuraminic Acid in axenic amastigotes. Promastigotes treated with sialidase infected less macrophages than parasites displaying total Sias. Our results demonstrate that Sias vary between Leishmania species and between L. (L.) amazonensis life stages and plays an important role in macrophage infection by L. (L.) amazonensis and L. (L.) infantum.

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亚马逊利什曼原虫和婴儿利什曼原虫唾液酸增强巨噬细胞感染

由利什曼原虫寄生虫引起的利什曼病影响着全世界数百万人。利什曼原虫是由白蛉亚科的雌性白蛉在吸食血液时传播的。在哺乳动物中，原无性体主要被巨噬细胞吞噬，分化为无性体并繁殖。为了进入巨噬细胞并存活，利什曼原虫利用诸如表面糖缀合物等毒力因子。唾液酸（Sias）存在于糖缀合物的末端，在人类病原体中起重要作用。Sias的重要性仅在非洲、亚洲和欧洲与内脏利什曼病有关的l (l) donovani中得到了探讨。因此，本研究的目的是表征亚马逊利什曼原虫（L.）和婴儿利什曼原虫（L.）的Sias，分别与南美洲皮肤和内脏利什曼原虫有关。为此，我们采用HPLC-FLD分析了亚马孙乳杆菌LV79株和2株婴儿乳杆菌的原乳糜虫数量，以及亚马孙乳杆菌无性系和感染小鼠足部损伤的无性系乳糜虫数量。为了评估Sias在promastigotes中的重要性，我们用唾液酸酶处理固定期寄生虫，并感染小鼠和人巨噬细胞。我们在所有菌株的前毛菌中检测到n -乙酰神经氨酸，其中在乳酸菌中含量较高。我们在脚爪损伤的无尾线虫中鉴定出n -乙酰神经氨酸和n -糖基神经氨酸，而在无尾线虫中仅鉴定出n -乙酰神经氨酸。唾液酸酶处理的原鞭毛虫感染的巨噬细胞比显示总Sias的寄生虫少。结果表明，Sias在不同利什曼原虫种类和不同亚马逊乳杆菌生命阶段存在差异，并在亚马逊乳杆菌和婴儿乳杆菌的巨噬细胞感染中发挥重要作用。

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来源期刊

Cell Biology International 生物-细胞生物学

CiteScore

7.60

自引率

0.00%

发文量

208

审稿时长

1 months

期刊介绍： Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.