Tainá Cavalcante, Antônio Moreira Marques, Mariana Medina Medeiros, Tania Carolina Reis, Daniel Quina, Bruna Cunha de Alencar, Giuseppe Palmisano, Beatriz Simonsen Stolf
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引用次数: 0
Abstract
Leishmaniases affect millions of people around the world, caused by Leishmania parasites. Leishmania are transmitted by female sandflies from Phlebotominae subfamily during their blood meals. In mammals, promastigotes are phagocytosed mainly by macrophages, differentiate into amastigotes and multiply. For entry and survival in macrophages, Leishmania uses virulence factors such as surface glycoconjugates. Sialic acids (Sias) are found in terminal portions of glycoconjugates and play important roles in human pathogens. The importance of Sias was explored only in L. (L.) donovani, associated with visceral leishmaniasis in Africa, Asia and Europe. Thus, the aim of this study was to characterize Sias of Leishmania (L.) amazonensis and Leishmania (L.) infantum, related to cutaneous and visceral leishmaniasis in South America, respectively. For that, we analyzed by HPLC-FLD the Sias of promastigotes of L. (L.) amazonensis LV79 and two L. (L.) infantum strains, and of L. (L.) amazonensis axenic amastigotes and amastigotes from paw lesions of infected mice. To evaluate Sias importance in promastigotes, we treated stationary phase parasites with sialidase and infected murine and human macrophages. We detected N-Acetylneuraminic Acid in promastigotes of all strains, with greater abundance in L. (L.) infantum. We identified N-Acetylneuraminic Acid and N-Glycolylneuraminic acid in amastigotes recovered from paw lesion, but only N-Acetylneuraminic Acid in axenic amastigotes. Promastigotes treated with sialidase infected less macrophages than parasites displaying total Sias. Our results demonstrate that Sias vary between Leishmania species and between L. (L.) amazonensis life stages and plays an important role in macrophage infection by L. (L.) amazonensis and L. (L.) infantum.
期刊介绍:
Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect.
These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.