Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

IF 5.6 1区 工程技术 Q1 ENGINEERING, GEOLOGICAL
Lingxiao Liu, Yaqing Gao, Hao Meng, Xinwen Cao, Zhanbin Wang, Bin Liu, Jia He
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引用次数: 0

Abstract

Enzyme-induced carbonate precipitation (EICP) is an appealing bio-cementation technology for soil improvement in geotechnical engineering. This study investigated the bio-reinforcement efficacy of sword bean crude urease (SWCU)-mediated EICP and the enhancement effect of various additives on it. A set of sand column specimens with different bio-cementation levels were prepared. Magnesium chloride, sucrose, xanthan gum, sisal fiber, calcite seeds, and skim milk powder were adopted for comparison. Bio-reinforcement efficacy was evaluated by mechanical properties. SWCU possessed a ~ 127% higher specific activity than entry-level commercial urease while saving over 2000 times the enzyme cost. All specimens treated with SWCU-mediated EICP presented excellent moldability and uniformity for one-time treatment. UCS increased exponentially with bio-cementation level due to the uniformly growing CaCO3 content and crystal size. UCS of ~ 1.8 MPa was achieved in a single treatment using 60 g/L SWCU and 3.0 M urea-CaCl2. SWCU exhibited a superior bio-reinforcement efficiency over soybean crude urease, commercial urease, and bacterial urease, since higher soil strength was achieved at lower CaCO3 content. Magnesium chloride showed the most significant enhancement effect, implying an extensive application prospect of SWCU-mediated EICP in seawater environments. The absence of wet strength, markedly elevated dry strength, and notably higher stiffness and hardness at low stress (load) phase indicated that xanthan gum would be more suitable for windbreak and sand fixation in arid/semi-arid environments. Sisal fiber could also effectively improve soil mechanical properties; however, the labor and time costs caused by its premixing with soil should be considered additionally in practical applications.

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来源期刊
Acta Geotechnica
Acta Geotechnica ENGINEERING, GEOLOGICAL-
CiteScore
9.90
自引率
17.50%
发文量
297
审稿时长
4 months
期刊介绍: Acta Geotechnica is an international journal devoted to the publication and dissemination of basic and applied research in geoengineering – an interdisciplinary field dealing with geomaterials such as soils and rocks. Coverage emphasizes the interplay between geomechanical models and their engineering applications. The journal presents original research papers on fundamental concepts in geomechanics and their novel applications in geoengineering based on experimental, analytical and/or numerical approaches. The main purpose of the journal is to foster understanding of the fundamental mechanisms behind the phenomena and processes in geomaterials, from kilometer-scale problems as they occur in geoscience, and down to the nano-scale, with their potential impact on geoengineering. The journal strives to report and archive progress in the field in a timely manner, presenting research papers, review articles, short notes and letters to the editors.
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