High-frequency ultrasound induced the preparation of oxidized low density lipoprotein

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-03-05 DOI:10.1016/j.ultsonch.2025.107303

Yuanmin Li , Wanyue Yang , Xinyi Zhang , Jingjing Ba , Han Yang , Wen Wang , Ke Zhang , Ze Yang , Hui Liang , Zihan Li , Muthupandian Ashokkumar , Jiguo Zhang , Zhiliang Gao , Yang Yu

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引用次数: 0

Abstract

Foam cells have been frequently used in studies related to atherosclerosis. Traditional methods for inducing oxidized low-density lipoprotein (oxLDL) involve copper ion (Cu²⁺) treatment, which has inherent limitations such as prolonged oxidation times and residual copper ions. This study explored high-frequency ultrasound (400 kHz) as an alternative method for LDL oxidization. The findings demonstrated that high-frequency ultrasound-oxidized LDL (U-oxLDL) exhibited no significant differences compared to copper-oxidized LDL (Cu-oxLDL) in terms of electrophoretic mobility, foam cell morphology, lipid content, and cholesterol transport proteins. Additionally, lipidomic analysis revealed that U-oxLDL was more comparable to native LDL (N-LDL). Transcriptomic profiling of bone marrow-derived macrophages (BMDMs) treated with oxLDL showed that the gene expression patterns of BMDM foam cells treated with U-oxLDL were over 90 % consistent with those treated with Cu-oxLDL. Therefore, high-frequency ultrasound oxidation method represents a green and efficient strategy for oxLDL preparation, offering potential advantages for advancing atherosclerosis research.

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高频超声诱导制备氧化低密度脂蛋白

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.