Lysine enhances the photoresponsive oxidase-like activity of twin Cd0.7Zn0.3S for direct colorimetric detection of lysine

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-03-07 DOI:10.1016/j.aca.2025.343914

Xiaorong Sun, Zhongfang Hu, Qing Zhou, Guang-Li Wang

{"title":"Lysine enhances the photoresponsive oxidase-like activity of twin Cd0.7Zn0.3S for direct colorimetric detection of lysine","authors":"Xiaorong Sun, Zhongfang Hu, Qing Zhou, Guang-Li Wang","doi":"10.1016/j.aca.2025.343914","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>Lysine (Lys) is one of the eight essential amino acids for the human body, which can't be synthesized by the body and must be obtained from external sources. And the detection of Lys is of significance for disease monitoring. The construction of photoresponsive nanozymes based analytical methods have received increasing attention and have been successfully achieved for the detection of metal ions, small molecules and natural enzymes. However, the exploration of photoresponsive nanozyme in amino acids detection has not been tapped.<h3>Results</h3>This study presents an innovative method based on surface passivation by Lys to stimulate the photoresponsive nanozyme activity of twin Cd<sub>0.7</sub>Zn<sub>0.3</sub>S nanomaterials. Specifically, Lys can bind with twin Cd<sub>0.7</sub>Zn<sub>0.3</sub>S, which filled the dangling bonds on the surface of Cd<sub>0.7</sub>Zn<sub>0.3</sub>S and caused passivation of the surface state, resulting in the promotion of the separation efficiency of electrons and holes, along with the facilitation of the production of active intermediates. Therefore, the Cd<sub>0.7</sub>Zn<sub>0.3</sub>S in the presence of Lys showed a high catalytic oxidation ability for the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB). This new kind of photoresponsive oxidase-like activity could be regulated by switching visible light sources and showed the specificity of being only affected by Lys without influenced by other amino acids, thus achieved direct colorimetric detection of Lys. The linear range for Lys detection was 1-100 μM, with a detection limit of 0.18 μM (S/N=3).<h3>Significance</h3>This study developed a new nanozyme of twin Cd<sub>0.7</sub>Zn<sub>0.3</sub>S, whose activity leverages on Lys as a stimulator. Moreover, the Lys detection method proposed by us had the characteristics of high sensitivity, good selectivity, fast detection speed, and low cost. Therefore, it held significant potential application value, making it a promising candidate in the field of Lys detection and related research areas.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"12 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2025.343914","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Lysine (Lys) is one of the eight essential amino acids for the human body, which can't be synthesized by the body and must be obtained from external sources. And the detection of Lys is of significance for disease monitoring. The construction of photoresponsive nanozymes based analytical methods have received increasing attention and have been successfully achieved for the detection of metal ions, small molecules and natural enzymes. However, the exploration of photoresponsive nanozyme in amino acids detection has not been tapped.

Results

This study presents an innovative method based on surface passivation by Lys to stimulate the photoresponsive nanozyme activity of twin Cd_0.7Zn_0.3S nanomaterials. Specifically, Lys can bind with twin Cd_0.7Zn_0.3S, which filled the dangling bonds on the surface of Cd_0.7Zn_0.3S and caused passivation of the surface state, resulting in the promotion of the separation efficiency of electrons and holes, along with the facilitation of the production of active intermediates. Therefore, the Cd_0.7Zn_0.3S in the presence of Lys showed a high catalytic oxidation ability for the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB). This new kind of photoresponsive oxidase-like activity could be regulated by switching visible light sources and showed the specificity of being only affected by Lys without influenced by other amino acids, thus achieved direct colorimetric detection of Lys. The linear range for Lys detection was 1-100 μM, with a detection limit of 0.18 μM (S/N=3).

Significance

This study developed a new nanozyme of twin Cd_0.7Zn_0.3S, whose activity leverages on Lys as a stimulator. Moreover, the Lys detection method proposed by us had the characteristics of high sensitivity, good selectivity, fast detection speed, and low cost. Therefore, it held significant potential application value, making it a promising candidate in the field of Lys detection and related research areas.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.