Transcriptome response of the white-rot fungus Trametes versicolor to hybrid poplar exhibiting unique lignin chemistry.

Q1 Agricultural and Biological Sciences
Anbarah R Alzabaidi, Noor Alabbasi, Richard Meilan, Scott J Meiners, Thomas Canam
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引用次数: 0

Abstract

Background: Production of biofuels and bioproducts from lignocellulosic material is limited due to the complexity of the cell wall structure. This necessitates the use of physical, chemical, and/or physico-chemical pretreatment technologies, which adds significant capital, operational, and environmental costs. Biological pretreatment strategies have the potential to mitigate these expenses by harnessing the innate ability of specialized bacteria and fungi to deconstruct lignocellulose. White-rot fungi (e.g. Trametes versicolor) have been shown to be effective at biological pretreatment of lignocellulose, yet it was uncertain if these fungi are feedstock agnostic or are able to sense subtle changes in cell wall chemistry.

Results: The present study examined the transcriptome response by Trametes versicolor to transgenic hybrid poplar (Populus tremula × alba) lines with altered syringyl (S) and guaiacyl (G) lignin. Specifically, the transcriptional response of the fungus to wild-type wood was compared to that from the wood of six transgenic lines within three lignin phenotypes, LSX (low S with hydroxy-G), LSHG (low S with high G), and HS (high S), with 350 transcripts showing significant differences among the samples. The transcriptome of T. versicolor varied according to the lignin phenotype of the wood, with the LSX wood resulting in the most substantial changes in T. versicolor transcript abundance. Specifically, the LSX wood led to 50 upregulated and 48 downregulated transcripts from WT at the twofold or greater threshold. For example, transcripts for the lignin peroxidases LiP3 and LiP10 were downregulated (approximately 12X and 31X lower, respectively) by the fungus on LSX wood compared to wild-type wood. LSX wood also resulted in approximately 11X lower transcript numbers of endo-β-1,4-glucanase yet led to an increase in expression of certain hemicellulases, further highlighting the altered deconstruction strategy by the fungus on this wood type.

Conclusions: Overall, the results of this study demonstrated that T. versicolor was able to respond to transgenic poplar wood with the same genetic background, which has important implications for biological pretreatment strategies involving feedstocks that are genetically modified or have considerable natural variations in cell wall chemistry.

白腐菌对杂交杨树表现出独特的木质素化学反应的转录组反应。
背景:由于细胞壁结构的复杂性,木质纤维素材料的生物燃料和生物产品的生产受到限制。这就需要使用物理、化学和/或物理化学预处理技术,这增加了大量的资金、操作和环境成本。生物预处理策略有可能通过利用特定细菌和真菌的固有能力来解构木质纤维素来减轻这些费用。白腐真菌(如花斑曲霉)已被证明在木质纤维素的生物预处理方面是有效的,但尚不确定这些真菌是否与原料无关,还是能够感知细胞壁化学的细微变化。结果:本研究检测了花叶曲霉(Trametes versicolor)对紫丁香基(S)和愈创木酰(G)木质素转基因杂交杨树(Populus tremula × alba)的转录组反应。具体来说,我们比较了该真菌对野生型木材的转录反应与来自6个木质素表型(LSX(低S带羟基G)、LSHG(低S带高G)和HS(高S)的木质素表型)的转录反应,其中350个转录本在样品之间存在显著差异。木质素表型不同,木质素转录组也不同,其中LSX木材对木质素转录组丰度的影响最为显著。具体来说,在两倍或更高的阈值下,LSX木材导致50个转录本上调,48个转录本下调。例如,与野生型木材相比,真菌对LSX木材上木质素过氧化物酶LiP3和LiP10的转录物下调(分别下调约12倍和31倍)。LSX木材还导致endo-β-1,4-葡聚糖酶的转录数降低约11X,但导致某些半纤维素酶的表达增加,进一步突出了真菌对这种木材类型的解构策略的改变。结论:总体而言,本研究结果表明,T. versicolor能够对具有相同遗传背景的转基因杨木产生反应,这对涉及转基因原料或细胞壁化学有较大自然变化的原料的生物预处理策略具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Fungal Biology and Biotechnology
Fungal Biology and Biotechnology Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
10.20
自引率
0.00%
发文量
17
审稿时长
9 weeks
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