Investigation and analysis of female HPV infection and genotype distribution in Xuhui District, Shanghai.

IF 4 3区 医学 Q2 VIROLOGY
Han Liu, Mingming Jiang, Jiaying Wu, Yue Dai, Minyi Xu, Lei Wang, Muyuan Ji
{"title":"Investigation and analysis of female HPV infection and genotype distribution in Xuhui District, Shanghai.","authors":"Han Liu, Mingming Jiang, Jiaying Wu, Yue Dai, Minyi Xu, Lei Wang, Muyuan Ji","doi":"10.1186/s12985-025-02663-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>In China, Cervical cancer is one of the common malignant tumors in females, and high-risk human papillomavirus (HR-HPV) infection is one of its main causative factors. However, human papillomavirus (HPV) infection rates may vary significantly among patients of different ages and HPV subtypes. This study aims to provide insights into developing cervical cancer screening strategies and selecting HPV vaccine antigen targets in the area.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the HPV testing results of 47,423 women from January 2017 to April 2023 at the Clinical Laboratory of the Eighth People's Hospital in Shanghai. HPV DNA genotyping was performed using real-time quantitative polymerase chain reaction (PCR) in the molecular laboratory. Statistical analysis was carried out using GraphPad Prism 8.0.1 software. Binomial distribution analysis was used to calculate the 95% confidence intervals (95% CI), and the chi-square test was employed to compare categorical variables among different age groups, with a p-value of less than 0.05 indicating statistical significance.</p><p><strong>Results: </strong>Among the 47,423 cervical HPV DNA test results, the overall infection rate was 18.9%, with single infections accounting for 13.93%, dual infections for 3.47%, and multiple infections for 1.5%. The age-specific prevalence of HPV infection exhibited a \"U\"-shaped curve, with the highest infection rates observed in the age groups under 30 and between 50 and 59 years. The five most common HR-HPV subtypes in Xuhui District were types 16, 39, 51, 52, 56, and 58 (accounting for 10.3%, 7%, 8%, 20.3%, 6%, and 12%, respectively), with type 52 showing the highest infection rate. The prevalence of moderate/severe HPV infection rates in the HPV 59, HPV 33, and HPV 35 gene subtypes increased over time, highlighting the importance of monitoring these subtypes.</p><p><strong>Conclusion: </strong>This study identified the primary HR-HPV genotypes prevalent among females in Xuhui District, Shanghai, and explored correlations between age, genotype, and HPV infection rates. While the findings provide a basis for recommending HPV screening for younger and older age groups, further studies integrating clinical outcomes such as cytological and pathological results are necessary to substantiate these conclusions and refine screening strategies. Due to variations in HPV trends globally and regional differences in genotypes, epidemiological analysis of HPV can accurately and visually reflect the distribution of specific HPV genotypes in a particular area, thereby aiding in the development of regional cervical cancer screening strategies and the selection of HPV vaccine antigen targets.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"59"},"PeriodicalIF":4.0000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881386/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12985-025-02663-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: In China, Cervical cancer is one of the common malignant tumors in females, and high-risk human papillomavirus (HR-HPV) infection is one of its main causative factors. However, human papillomavirus (HPV) infection rates may vary significantly among patients of different ages and HPV subtypes. This study aims to provide insights into developing cervical cancer screening strategies and selecting HPV vaccine antigen targets in the area.

Methods: A retrospective analysis was conducted on the HPV testing results of 47,423 women from January 2017 to April 2023 at the Clinical Laboratory of the Eighth People's Hospital in Shanghai. HPV DNA genotyping was performed using real-time quantitative polymerase chain reaction (PCR) in the molecular laboratory. Statistical analysis was carried out using GraphPad Prism 8.0.1 software. Binomial distribution analysis was used to calculate the 95% confidence intervals (95% CI), and the chi-square test was employed to compare categorical variables among different age groups, with a p-value of less than 0.05 indicating statistical significance.

Results: Among the 47,423 cervical HPV DNA test results, the overall infection rate was 18.9%, with single infections accounting for 13.93%, dual infections for 3.47%, and multiple infections for 1.5%. The age-specific prevalence of HPV infection exhibited a "U"-shaped curve, with the highest infection rates observed in the age groups under 30 and between 50 and 59 years. The five most common HR-HPV subtypes in Xuhui District were types 16, 39, 51, 52, 56, and 58 (accounting for 10.3%, 7%, 8%, 20.3%, 6%, and 12%, respectively), with type 52 showing the highest infection rate. The prevalence of moderate/severe HPV infection rates in the HPV 59, HPV 33, and HPV 35 gene subtypes increased over time, highlighting the importance of monitoring these subtypes.

Conclusion: This study identified the primary HR-HPV genotypes prevalent among females in Xuhui District, Shanghai, and explored correlations between age, genotype, and HPV infection rates. While the findings provide a basis for recommending HPV screening for younger and older age groups, further studies integrating clinical outcomes such as cytological and pathological results are necessary to substantiate these conclusions and refine screening strategies. Due to variations in HPV trends globally and regional differences in genotypes, epidemiological analysis of HPV can accurately and visually reflect the distribution of specific HPV genotypes in a particular area, thereby aiding in the development of regional cervical cancer screening strategies and the selection of HPV vaccine antigen targets.

研究目的在中国,宫颈癌是女性常见的恶性肿瘤之一,而高危人乳头瘤病毒(HR-HPV)感染是其主要致病因素之一。然而,人乳头瘤病毒(HPV)感染率在不同年龄、不同 HPV 亚型的患者中可能存在显著差异。本研究旨在为该地区制定宫颈癌筛查策略和选择 HPV 疫苗抗原靶点提供见解:方法:对上海市第八人民医院临床检验科 2017 年 1 月至 2023 年 4 月期间 47,423 名女性的 HPV 检测结果进行回顾性分析。分子实验室使用实时定量聚合酶链反应(PCR)进行HPV DNA基因分型。使用 GraphPad Prism 8.0.1 软件进行统计分析。采用二项分布分析法计算95%置信区间(95% CI),采用卡方检验法比较不同年龄组的分类变量,P值小于0.05表示有统计学意义:在 47 423 份宫颈 HPV DNA 检测结果中,总体感染率为 18.9%,其中单感染占 13.93%,双感染占 3.47%,多感染占 1.5%。HPV感染的年龄特异性流行率呈 "U "形曲线,30岁以下和50至59岁年龄组的感染率最高。徐汇区最常见的五种 HR-HPV 亚型分别为 16、39、51、52、56 和 58 型(分别占 10.3%、7%、8%、20.3%、6% 和 12%),其中 52 型的感染率最高。随着时间的推移,HPV 59、HPV 33 和 HPV 35 基因亚型的中度/重度 HPV 感染率有所上升,这凸显了监测这些亚型的重要性:本研究确定了上海徐汇区女性中流行的主要 HR-HPV 基因型,并探讨了年龄、基因型和 HPV 感染率之间的相关性。虽然研究结果为建议对年轻和年长群体进行HPV筛查提供了依据,但仍有必要结合细胞学和病理学结果等临床结果开展进一步研究,以证实这些结论并完善筛查策略。由于全球 HPV 趋势的变化和基因型的地区差异,HPV 流行病学分析可以准确、直观地反映特定地区特定 HPV 基因型的分布情况,从而有助于制定地区性宫颈癌筛查策略和选择 HPV 疫苗抗原靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Virology Journal
Virology Journal 医学-病毒学
CiteScore
7.40
自引率
2.10%
发文量
186
审稿时长
1 months
期刊介绍: Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信