Fast screening libraries of plant growth promoting rhizobacteria (PGPRs) for insecticidal activity.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-03-03 DOI:10.1093/jambio/lxaf054

Richard O Murphy, Janiyah S Cotton, Isabella M Owens, Jazmine D Carroll, Kathleen M Martin, David Held, Kathy Lawrence, John F Beckmann

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引用次数: 0

Abstract

Aims: Plant growth-promoting rhizobacteria (PGPR) are ubiquitous among soil bacteria and aid in many growth-related functions for plants. Recent biotechnological advancements involving PGPRs have led to a growing desire to explore and uncover combinatorial or dual use PGPRs. Our study aimed to discover novel insecticidal phenotypes amongst PGPR libraries.

Methods and results: We screened, through survivorship bioassays, 502 endophytic PGPRs for insecticidal activity against Drosophila melanogaster (fruit flies). Initial bioassay screens were followed by higher (n) testing and subsequent insecticidal assays against 4 key pest species including D. suzukii, Spodoptera frugiperda, Aphis gossypii, and Lyguslineolaris. Of the 502 endophytic PGPRs screened, 39 strains showed statistically significant mortality against insects with three strains killing four pest species and seven killing three pest species. The key insecticidal strains primarily consisted of Serratia marcescens and Bacillus velezensis.

Conclusions: In total, ∼8% of strains tested showed insecticidal phenotypes. The fast screening methodology was successful in uncovering dual use PGPRs. Some bacteria were broad spectrum insect killers and others were more specific to a particular pest.

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.