Identification and function analysis of two new gustatory receptors related to silkworm monophagy.

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY
Jian-Zhao Jiang, Qin Xiao, Zhuo-Lin Gao, Jun Gu, Li-Hua Huang
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Abstract

Silkworm is a typical monophagous insect that can only feed on fresh mulberry leaves. The mechanism for this monophagous nature is not fully understood. One bitter gustatory receptor (GR) GR66 located on the maxilla of the mouthpart has been reported to be an important factor influencing the feeding preference of silkworm. However, the preference of GR66 mutants for a nonhost plant was very low, suggesting that other factors related to silkworm monophagy need to be further explored. In this study, 10 bitter GRs were screened out based on their specific high expression in the maxilla of silkworm, and the 4 most expressed GRs (GR15, GR43, GR69, and GR66) were knocked out by clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9. Feeding experiments showed that except for GR69-/-, the host range of GR43-/-, GR15-/-, and GR66-/- expanded remarkably, and both GR43-/- and GR66-/- revealed the highest preference for the nonhost plants. Moreover, the strict preference for mulberry leaves was almost eliminated in the double mutant silkworms of GR43-/- and GR66-/-. These results imply that it is likely that a variety of different bitter GRs are involved in determining the monophagy of silkworm larvae. In addition, GR15 mutation significantly inhibited ovarian development, resulting in a significant decrease in oviposition. These findings enhance our understanding of the monophagous nature of silkworms and provide a possibility for the molecular breeding of polyphagous silkworms in the future.

家蚕单食相关的两种新的味觉受体鉴定及功能分析。
蚕是一种典型的单食昆虫,只能以新鲜的桑叶为食。这种单食性的机制尚不完全清楚。据报道,家蚕口器上的一个苦味感受器GR66是影响其摄食偏好的重要因素。然而,GR66突变体对一种非寄主植物的偏好度很低,表明与家蚕单食性有关的其他因素有待进一步探索。本研究根据家蚕上颌骨中特异性高表达的基因筛选出10个苦味基因,并通过聚集规则间隔短回文重复序列(CRISPR) / CRISPR相关核酸酶9敲除表达最多的4个基因(GR15、GR43、GR69和GR66)。取食实验表明,除GR69-/-外,GR43-/-、GR15-/-和GR66-/-的寄主范围显著扩大,且GR43-/-和GR66-/-对非寄主植物的偏好程度最高。此外,GR43-/-和GR66-/-双突变家蚕对桑叶的严格偏好几乎被消除。这些结果表明,蚕幼虫的单食性可能与多种不同的苦味gr有关。此外,GR15突变显著抑制卵巢发育,导致排卵显著减少。这些发现增加了我们对家蚕单食性的认识,并为今后多食家蚕的分子育种提供了可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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