{"title":"Advancements in extracellular vesicles biomanufacturing: a comprehensive overview of large-scale production and clinical research.","authors":"Ziqian Li, Junyu Yan, Xiang Li, Hui Chen, Chen Lin, Yuhang Zhang, Tian Gao, Yabo Zhang, Yue Shu, Shuyuan Pan, Yuntao Zhang","doi":"10.3389/fbioe.2025.1487627","DOIUrl":null,"url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in the field of biosciences for their role in intercellular communication in various physiological and pathological processes. Their ubiquitous presence in bodily fluids and cell-specific characteristics make them promising candidates as biomarkers. Additionally, their ability to transport biological therapeutics across different biological barriers to specific target cells underscores their significant translational potential for diagnostic and therapeutic purposes. Significant progress has been achieved in the translation of EVs research to clinical applications, however, challenges persist in the large-scale production of EVs, particularly in the areas of scalable manufacturing, efficient isolation methods, drug loading techniques, and advanced characterization technology. This review critically examines the complex processes involved in EVs biogenesis and explores recent developments in large-scale EVs production. By synthesizing knowledge from these fields, this review aims to provide a holistic perspective on the evolving landscape of EVs research and its applications, underscoring both the accomplishments and the obstacles that lie ahead in fully realizing the potential of EVs in biomedicine.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1487627"},"PeriodicalIF":4.3000,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879961/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Bioengineering and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3389/fbioe.2025.1487627","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in the field of biosciences for their role in intercellular communication in various physiological and pathological processes. Their ubiquitous presence in bodily fluids and cell-specific characteristics make them promising candidates as biomarkers. Additionally, their ability to transport biological therapeutics across different biological barriers to specific target cells underscores their significant translational potential for diagnostic and therapeutic purposes. Significant progress has been achieved in the translation of EVs research to clinical applications, however, challenges persist in the large-scale production of EVs, particularly in the areas of scalable manufacturing, efficient isolation methods, drug loading techniques, and advanced characterization technology. This review critically examines the complex processes involved in EVs biogenesis and explores recent developments in large-scale EVs production. By synthesizing knowledge from these fields, this review aims to provide a holistic perspective on the evolving landscape of EVs research and its applications, underscoring both the accomplishments and the obstacles that lie ahead in fully realizing the potential of EVs in biomedicine.
期刊介绍：
The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs.
In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.
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