Influence of Injectable Hyaluronic Gel System on Skin Microbiota, Skin Defense Mechanisms and Integrity (Ex vivo Study).

IF 1.9 4区医学 Q3 DERMATOLOGY

Clinical, Cosmetic and Investigational Dermatology Pub Date : 2025-03-01 eCollection Date: 2025-01-01 DOI:10.2147/CCID.S491685

Aleksei Y Prokopov, Zarema I Gazitaeva, Anna N Sidorina, Laurent Peno-Mazzarino, Nikita Radionov, Anna O Drobintseva, Igor M Kvetnoy

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引用次数: 0

Abstract

Objective: The influence of injectable hyaluronic gels on skin's microbiota is unclear. As well, skin microbiota is a key factor modulating final effect of injectable gels. The ex-vivo study was aimed at alterations following hyaluronic acid injection into the dermis in non-sterile skin surface conditions.

Methods: Ex vivo human skin explants in the presence or absence of either S. epidermidis or S. aureus, were treated with either control excipient (0.9% sodium chloride) or test product (Hyaluronic acid injectable S, HA-S). Bacterial analysis was performed, as well as skin structural integrity. Histological imaging and immunostaining analysis in the presence of skin markers: epidermal (CD1a, Toll-like receptor 2 (TLR2), Beta-defensin-3 (BD3), CCN1) and dermal (DC-SIGN, Decorin) were then performed.

Results: The injection of control excipient E and test product P, both associated with bacterial deposits, induced similar noticeable increase of S. epidermidis growth over 4 days, but no noticeable effect on growth of S. aureus. The injection of control excipient, associated with bacterial deposits, showed epidermal and dermal alterations increased with time. It was observed significant increase of epidermal CD1a, TLR2, CCN1 and dermal DC-SIGN, Decorin on Day 2. The injection of test product, associated with bacterial deposits, in contrast to injection of control excipient, associated with bacterial deposits, induced very slight but significant improvement of epidermal viability as well as significant decrease of epidermal TLR2, BD3, CCN1 and dermal DC-SIGN on Day 2.

Conclusion: Our investigation showed that both intradermal injections, HA-based solution or control excipient, trigger short-term skin microbiota growth. We indicate strong influence of non-sterile skin surface conditions on human skin explant viability when skin barrier damaged by injection puncture and highlights differences of epidermal/dermal response depended on injected composition.

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注射透明质酸凝胶系统对皮肤微生物群、皮肤防御机制和完整性的影响（离体研究）。

目的：目前尚不清楚注射透明质酸凝胶对皮肤微生物群的影响。此外，皮肤微生物群是调节注射凝胶最终效果的关键因素。离体研究的目的是在非无菌皮肤表面条件下将透明质酸注射到真皮层后的变化。方法：用对照辅料（0.9%氯化钠）或试验品（透明质酸注射用S， HA-S）处理存在或不存在表皮葡萄球菌或金黄色葡萄球菌的离体人皮肤外植体。进行细菌分析，以及皮肤结构完整性。在皮肤标记物存在的情况下进行组织学成像和免疫染色分析：表皮(CD1a， toll样受体2 （TLR2)， β -防御素-3 (BD3), CCN1）和真皮（DC-SIGN, Decorin）。结果：对照辅料E和试验品P均与细菌沉积有关，在4 d内均可诱导表皮葡萄球菌生长明显增加，但对金黄色葡萄球菌生长无明显影响。与细菌沉积相关的对照辅料的注射显示表皮和真皮的改变随着时间的推移而增加。第2天表皮CD1a、TLR2、CCN1及真皮DC-SIGN、Decorin均显著升高。注射与细菌沉积相关的试验产品，与注射与细菌沉积相关的对照辅料相比，在第2天，表皮活力得到了非常轻微但显著的改善，表皮TLR2、BD3、CCN1和真皮DC-SIGN也显著降低。结论：我们的研究表明皮内注射、ha溶液或对照赋形剂都能引发皮肤微生物群的短期生长。我们指出，当皮肤屏障被注射穿刺破坏时，非无菌皮肤表面条件对人体皮肤外植体存活能力有很大影响，并强调了不同注射成分对表皮/真皮反应的差异。

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来源期刊

Clinical, Cosmetic and Investigational Dermatology Medicine-Dermatology

CiteScore

2.80

自引率

4.30%

发文量

353

审稿时长

16 weeks

期刊介绍： Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal. Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest. The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care. All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.