Influence of Injectable Hyaluronic Gel System on Skin Microbiota, Skin Defense Mechanisms and Integrity (Ex vivo Study).

IF 1.9 4区医学 Q3 DERMATOLOGY

Clinical, Cosmetic and Investigational Dermatology Pub Date : 2025-03-01 eCollection Date: 2025-01-01 DOI:10.2147/CCID.S491685

Aleksei Y Prokopov, Zarema I Gazitaeva, Anna N Sidorina, Laurent Peno-Mazzarino, Nikita Radionov, Anna O Drobintseva, Igor M Kvetnoy

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引用次数: 0

Abstract

Objective: The influence of injectable hyaluronic gels on skin's microbiota is unclear. As well, skin microbiota is a key factor modulating final effect of injectable gels. The ex-vivo study was aimed at alterations following hyaluronic acid injection into the dermis in non-sterile skin surface conditions.

Methods: Ex vivo human skin explants in the presence or absence of either S. epidermidis or S. aureus, were treated with either control excipient (0.9% sodium chloride) or test product (Hyaluronic acid injectable S, HA-S). Bacterial analysis was performed, as well as skin structural integrity. Histological imaging and immunostaining analysis in the presence of skin markers: epidermal (CD1a, Toll-like receptor 2 (TLR2), Beta-defensin-3 (BD3), CCN1) and dermal (DC-SIGN, Decorin) were then performed.

Results: The injection of control excipient E and test product P, both associated with bacterial deposits, induced similar noticeable increase of S. epidermidis growth over 4 days, but no noticeable effect on growth of S. aureus. The injection of control excipient, associated with bacterial deposits, showed epidermal and dermal alterations increased with time. It was observed significant increase of epidermal CD1a, TLR2, CCN1 and dermal DC-SIGN, Decorin on Day 2. The injection of test product, associated with bacterial deposits, in contrast to injection of control excipient, associated with bacterial deposits, induced very slight but significant improvement of epidermal viability as well as significant decrease of epidermal TLR2, BD3, CCN1 and dermal DC-SIGN on Day 2.

Conclusion: Our investigation showed that both intradermal injections, HA-based solution or control excipient, trigger short-term skin microbiota growth. We indicate strong influence of non-sterile skin surface conditions on human skin explant viability when skin barrier damaged by injection puncture and highlights differences of epidermal/dermal response depended on injected composition.

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来源期刊

Clinical, Cosmetic and Investigational Dermatology Medicine-Dermatology

CiteScore

2.80

自引率

4.30%

发文量

353

审稿时长

16 weeks

期刊介绍： Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal. Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest. The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care. All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.